CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019538
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pseudouridylate synthase 7 homolog 
Protein Synonyms/Alias
  
Gene Name
 PUS7 
Gene Synonyms/Alias
 KIAA1897 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
131QGFSGILKERYSDFVacetylation[1]
176TVLTAEEKQRLEELQubiquitination[2]
278DAINVLSKYLRVKPNubiquitination[3, 4, 5]
318QRLAHLNKCLMNFKLubiquitination[3, 5, 6]
324NKCLMNFKLGNFSYQubiquitination[3, 5, 6]
332LGNFSYQKNPLKLGEubiquitination[6]
415KPRSGAEKGYLVKCRubiquitination[6]
609DVDNLEGKTPPVFASubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 DOMAIN 370 580 TRUD.
 ACT_SITE 294 294 Nucleophile (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 610 610 Phosphothreonine.  
Keyword
 Acetylation; Complete proteome; Isomerase; Phosphoprotein; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 661 AA 
Protein Sequence
MEMTEMTGVS LKRGALVVED NDSGVPVEET KKQKLSECSL TKGQDGLQND FLSISEDVPR 60
PPDTVSTGKG GKNSEAQLED EEEEEEDGLS EECEEEESES FADMMKHGLT EADVGITKFV 120
SSHQGFSGIL KERYSDFVVH EIGKDGRISH LNDLSIPVDE EDPSEDIFTV LTAEEKQRLE 180
ELQLFKNKET SVAIEVIEDT KEKRTIIHQA IKSLFPGLET KTEDREGKKY IVAYHAAGKK 240
ALANPRKHSW PKSRGSYCHF VLYKENKDTM DAINVLSKYL RVKPNIFSYM GTKDKRAITV 300
QEIAVLKITA QRLAHLNKCL MNFKLGNFSY QKNPLKLGEL QGNHFTVVLR NITGTDDQVQ 360
QAMNSLKEIG FINYYGMQRF GTTAVPTYQV GRAILQNSWT EVMDLILKPR SGAEKGYLVK 420
CREEWAKTKD PTAALRKLPV KRCVEGQLLR GLSKYGMKNI VSAFGIIPRN NRLMYIHSYQ 480
SYVWNNMVSK RIEDYGLKPV PGDLVLKGAT ATYIEEDDVN NYSIHDVVMP LPGFDVIYPK 540
HKIQEAYREM LTADNLDIDN MRHKIRDYSL SGAYRKIIIR PQNVSWEVVA YDDPKIPLFN 600
TDVDNLEGKT PPVFASEGKY RALKMDFSLP PSTYATMAIR EVLKMDTSIK NQTQLNTTWL 660
R 661 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR001656; PsdUridine_synth_TruD.
 IPR020119; PsdUridine_synth_TruD_CS.
 IPR017091; PsdUridine_synth_TruD_euk.
 IPR011760; PsdUridine_synth_TruD_insert. 
Pfam
 PF01142; TruD 
SMART
  
PROSITE
 PS50984; TRUD
 PS01268; UPF0024 
PRINTS