CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024662
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding protein 25 
Protein Synonyms/Alias
 RNA-binding motif protein 25 
Gene Name
 Rbm25 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
125RVQGASGKLQAFGFCacetylation[1]
135AFGFCEYKEPESTLRacetylation[1, 2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA (By similarity). 
Sequence Annotation
 DOMAIN 87 164 RRM.
 DOMAIN 745 838 PWI.
 REGION 284 639 Necessary for nuclear speckle
 MOD_RES 578 578 Phosphoserine.
 MOD_RES 672 672 Phosphoserine.
 MOD_RES 678 678 Phosphoserine (By similarity).
 MOD_RES 698 698 Phosphoserine.
 CROSSLNK 472 472 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Apoptosis; Complete proteome; Cytoplasm; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 838 AA 
Protein Sequence
MSFPPHLNRP PMGIPALPPG IPPPQFPGFP PPVPPGTPMI PVPMSIMAPA PTVLVPTVSM 60
VGKHLGARKD HPGLKLKEND ENCGPTTTVF VGNISEKASD MLIRQLLAKC GLVLSWKRVQ 120
GASGKLQAFG FCEYKEPEST LRALRLLHDL QIGEKKLLVK VDAKTKAQLD EWKAKKKANG 180
NARPETVTND DEEALDEETK RRDQMIKGAI EVLIREYSSE LNAPSQESDS HPRKKKKEKK 240
EDIFRRFPVA PLIPYPLITK EDINAIEMEE DKRDLISREI SKFRDTHKKL EEEKGKKEKE 300
RQEIEKERRE RERERERERE RRERERERER EREREKEKER ERERERDRDR DRTKERDRDR 360
ERDRDRDRER SSDRNKDRSR SREKSRDRER ERERERERER ERERERERER ERERERERER 420
EKDKKRDREE DEEDAYERRK LERKLREKEA AYQERLKNWE IRERKKTREY EKEAEREEER 480
RREMAKEAKR LKEFLEDYDD DRDDPKYYRG SALQKRLRDR EKEMEADERD RKREKEELEE 540
IRQRLLAEGH PDPDAELQRM EQEAERRRQP QIKQEPESEE EEEEKQEKEE KREEPVEEEE 600
EPEQKPCLKP TLRPISSAPS VSSASGNATP NTPGDESPCG IIIPHENSPD QQQPEEHRPK 660
IGLSLKLGAS NSPGQPNSVK RKKLPVDSVF NKFEDEDSDD VPRKRKLVPL DYGEDDKNAT 720
KGTVNTEEKR KHIKSLIEKI PTAKPELFAY PLDWSIVDSI LMERRIRPWI NKKIIEYIGE 780
EEATLVDFVC SKVMAHSSPQ SILDDVAMVL DEEAEVFIVK MWRLLIYETE AKKIGLVK 838 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; ISS:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0097194; P:execution phase of apoptosis; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR002483; PWI_dom.
 IPR000504; RRM_dom. 
Pfam
 PF01480; PWI
 PF00076; RRM_1 
SMART
 SM00311; PWI
 SM00360; RRM 
PROSITE
 PS51025; PWI
 PS50102; RRM 
PRINTS