UniProt Accession

 UBP10_MOUSE; P52479; Q3T9L4; Q3TNN5; Q3TZB8; Q3U5E0; Q6ZQG9; Q91VY7 

Genbank Protein ID

 D84096; AK129083; AK153675; AK157970; AK165156; AK172443; CH466525; BC007134 

Genbank Nucleotide ID

 BAA12220.1; BAC97893.1; BAE32139.1; BAE34291.1; BAE38053.1; BAE43006.1; EDL11620.1; AAH07134.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase 10 

Protein Synonyms/Alias

 Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10 

Gene Name

 Usp10 

Gene Synonyms/Alias

 Kiaa0190; Ode-1; Uchrp 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
402	ETVTLIHKPVSLQPR	ubiquitination	[1]
671	SRRVTLEKLPPVLVL	ubiquitination	[1]
696	GGCQKLVKNIDYPVD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling (By similarity). 

Sequence Annotation

 REGION 2 99 Interaction with p53/TP53 (By
 ACT_SITE 418 418 Nucleophile (By similarity).
 ACT_SITE 743 743 Proton acceptor (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 24 24 Phosphothreonine (By similarity).
 MOD_RES 205 205 Phosphothreonine.
 MOD_RES 223 223 Phosphoserine (By similarity).
 MOD_RES 330 330 Phosphoserine; by ATM (By similarity).
 MOD_RES 359 359 Phosphoserine (By similarity).
 MOD_RES 364 364 Phosphoserine (By similarity).
 MOD_RES 570 570 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; DNA damage; DNA repair; Endosome; Hydrolase; Nucleus; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 792 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; ISS:UniProtKB.
 GO:0045111; C:intermediate filament cytoskeleton; IEA:Compara.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB.
 GO:0044325; F:ion channel binding; ISS:UniProtKB.
 GO:0002039; F:p53 binding; ISS:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR009818; Ataxin-2_C.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 

Pfam

 PF07145; PAM2
 PF00443; UCH 

SMART

  

PROSITE

 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 

PRINTS