CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018340
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U4/U6 small nuclear ribonucleoprotein Prp31 
Protein Synonyms/Alias
 Pre-mRNA-processing factor 31; Serologically defined breast cancer antigen NY-BR-99; U4/U6 snRNP 61 kDa protein; Protein 61K; hPrp31 
Gene Name
 PRPF31 
Gene Synonyms/Alias
 PRP31 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73KIEEYISKQAKASEVubiquitination[1]
76EYISKQAKASEVMGPubiquitination[1]
140LDYIRTVKELGNSLDubiquitination[1]
298AARLVAAKCTLAARVubiquitination[1]
321GKVGYELKDEIERKFubiquitination[1]
401FSLGHLGKSGSGRVRubiquitination[1]
417TQVNEATKARISKTLubiquitination[2]
430TLQRTLQKQSVVYGGubiquitination[2]
438QSVVYGGKSTIRDRSubiquitination[1]
470VNPQAAEKKVAEANQubiquitination[1]
471NPQAAEKKVAEANQKubiquitination[1]
478KVAEANQKYFSSMAEubiquitination[2]
490MAEFLKVKGEKSGLMubiquitination[1]
493FLKVKGEKSGLMST*ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Involved in pre-mRNA splicing. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. 
Sequence Annotation
 DOMAIN 215 333 Nop.
 REGION 351 364 Nuclear localization signal (NLS).
 MOD_RES 379 379 Phosphoserine.
 MOD_RES 450 450 Phosphoserine.
 MOD_RES 455 455 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Disease mutation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Retinitis pigmentosa; Ribonucleoprotein; RNA-binding; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF 60
AEIMMKIEEY ISKQAKASEV MGPVEAAPEY RVIVDANNLT VEIENELNII HKFIRDKYSK 120
RFPELESLVP NALDYIRTVK ELGNSLDKCK NNENLQQILT NATIMVVSVT ASTTQGQQLS 180
EEELERLEEA CDMALELNAS KHRIYEYVES RMSFIAPNLS IIIGASTAAK IMGVAGGLTN 240
LSKMPACNIM LLGAQRKTLS GFSSTSVLPH TGYIYHSDIV QSLPPDLRRK AARLVAAKCT 300
LAARVDSFHE STEGKVGYEL KDEIERKFDK WQEPPPVKQV KPLPAPLDGQ RKKRGGRRYR 360
KMKERLGLTE IRKQANRMSF GEIEEDAYQE DLGFSLGHLG KSGSGRVRQT QVNEATKARI 420
SKTLQRTLQK QSVVYGGKST IRDRSSGTAS SVAFTPLQGL EIVNPQAAEK KVAEANQKYF 480
SSMAEFLKVK GEKSGLMST 499 
Gene Ontology
 GO:0015030; C:Cajal body; IDA:MGI.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0005684; C:U2-type spliceosomal complex; IC:BHF-UCL.
 GO:0005687; C:U4 snRNP; IDA:BHF-UCL.
 GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:MGI.
 GO:0005690; C:U4atac snRNP; TAS:BHF-UCL.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0070990; F:snRNP binding; IPI:BHF-UCL.
 GO:0000244; P:assembly of spliceosomal tri-snRNP; IMP:BHF-UCL. 
Interpro
 IPR002687; Nop_dom.
 IPR012976; NOSIC.
 IPR027105; Prp31.
 IPR019175; Prp31_C. 
Pfam
 PF01798; Nop
 PF08060; NOSIC
 PF09785; Prp31_C 
SMART
 SM00931; NOSIC 
PROSITE
 PS51358; NOP 
PRINTS