CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024046
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SEC23-interacting protein 
Protein Synonyms/Alias
 p125 
Gene Name
 SEC23IP 
Gene Synonyms/Alias
 MSTP053 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
329YLYDRIRKAAYWEEEubiquitination[1, 2]
348RRCTWFYKGDTDSRFubiquitination[2]
365YTEEFSEKLEAEYKKubiquitination[2]
477DFRVVSLKLLRTHFKubiquitination[2, 3, 4]
491KKSLDDGKVSRVEFLubiquitination[2]
703IANFVEHKAAKLKKAubiquitination[2]
706FVEHKAAKLKKAASEubiquitination[2]
723AVAATSTKGQEQSAQubiquitination[1, 2]
746PSESNEPKRKLPVGAubiquitination[1]
815NYSLPTCKGFFNIYHubiquitination[2]
860KRLHLELKESLSRMGubiquitination[2]
871SRMGSDLKQGFISSLubiquitination[2]
879QGFISSLKSAWQTLNubiquitination[1]
910EKVANQIKEEEEKQVubiquitination[1]
931VESPDFSKDEDYLGKubiquitination[1]
938KDEDYLGKVGMLNGGubiquitination[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a role in the organization of endoplasmic reticulum exit sites. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinositol 5-phosphate (PI(5)P). 
Sequence Annotation
 DOMAIN 644 707 SAM.
 DOMAIN 779 989 DDHD.
 REGION 1 367 Interaction with SEC23A.
 MOD_RES 926 926 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Endoplasmic reticulum; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1000 AA 
Protein Sequence
MAERKPNGGS GGASTSSSGT NLLFSSSATE FSFNVPFIPV TQASASPASL LLPGEDSTDV 60
GEEDSFLGQT SIHTSAPQTF SYFSQVSSSS DPFGNIGQSP LTTAATSVGQ SGFPKPLTAL 120
PFTTGSQDVS NAFSPSISKA QPGAPPSSLM GINSYLPSQP SSLPPSYFGN QPQGIPQPGY 180
NPYRHTPGSS RANPYIAPPQ LQQCQTPGPP AHPPPSGPPV QMYQMPPGSL PPVPSSVQSP 240
AQQQVPARPG APSVQVPSPF LLQNQYEPVQ PHWFYCKEVE YKQLWMPFSV FDSLNLEEIY 300
NSVQPDPESV VLGTDGGRYD VYLYDRIRKA AYWEEEPAEV RRCTWFYKGD TDSRFIPYTE 360
EFSEKLEAEY KKAVTTNQWH RRLEFPSGET IVMHNPKVIV QFQPSSVPDE WGTTQDGQTR 420
PRVVKRGIDD NLDEIPDGEM PQVDHLVFVV HGIGPVCDLR FRSIIECVDD FRVVSLKLLR 480
THFKKSLDDG KVSRVEFLPV HWHSSLGGDA TGVDRNIKKI TLPSIGRFRH FTNETLLDIL 540
FYNSPTYCQT IVEKVGMEIN HLHALFMSRN PDFKGGVSVA GHSLGSLILF DILSNQKDLN 600
LSKCPGPLAV ANGVVKQLHF QEKQMPEEPK LTLDESYDLV VENKEVLTLQ ETLEALSLSE 660
YFSTFEKEKI DMESLLMCTV DDLKEMGIPL GPRKKIANFV EHKAAKLKKA ASEKKAVAAT 720
STKGQEQSAQ KTKDMASLPS ESNEPKRKLP VGACVSSVCV NYESFEVGAG QVSVAYNSLD 780
FEPEIFFALG SPIAMFLTIR GVDRIDENYS LPTCKGFFNI YHPLDPVAYR LEPMIVPDLD 840
LKAVLIPHHK GRKRLHLELK ESLSRMGSDL KQGFISSLKS AWQTLNEFAR AHTSSTQLQE 900
ELEKVANQIK EEEEKQVVEA EKVVESPDFS KDEDYLGKVG MLNGGRRIDY VLQEKPIESF 960
NEYLFALQSH LCYWESEDTA LLLLKEIYRT MNISPEQPQH 1000 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; TAS:ProtInc.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0007030; P:Golgi organization; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; TAS:ProtInc. 
Interpro
 IPR004177; DDHD.
 IPR001660; SAM.
 IPR013761; SAM/pointed.
 IPR021129; SAM_type1. 
Pfam
 PF02862; DDHD
 PF00536; SAM_1 
SMART
 SM00454; SAM 
PROSITE
 PS51043; DDHD
 PS50105; SAM_DOMAIN 
PRINTS