UniProt Accession

 TBCA_HUMAN; O75347 

Genbank Protein ID

 AF038952; BT007354; BC018210 

Genbank Nucleotide ID

 AAC39866.1; AAP36018.1; AAH18210.1 

Protein Name

 Tubulin-specific chaperone A 

Protein Synonyms/Alias

 TCP1-chaperonin cofactor A; Tubulin-folding cofactor A; CFA 

Gene Name

 TBCA 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	DPRVRQIKIKTGVVK	ubiquitination	[1]
28	KEKVMYEKEAKQQEE	acetylation	[2]
28	KEKVMYEKEAKQQEE	ubiquitination	[1]
31	VMYEKEAKQQEEKIE	ubiquitination	[1]
51	DGENYDIKKQAEILQ	ubiquitination	[1, 3, 4, 5, 6]
52	GENYDIKKQAEILQE	ubiquitination	[1, 3, 4]
86	QRILENEKDLEEAEE	ubiquitination	[4]
95	LEEAEEYKEARLVLD	ubiquitination	[4]
105	RLVLDSVKLEA****	ubiquitination	[1, 4, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Tubulin-folding protein; involved in the early step of the tubulin folding pathway. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 3D-structure; Acetylation; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 108 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; TAS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0051087; F:chaperone binding; TAS:ProtInc.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
 GO:0007023; P:post-chaperonin tubulin folding pathway; TAS:ProtInc.
 GO:0007021; P:tubulin complex assembly; IEA:InterPro. 

Interpro

 IPR004226; CofA_tubulin-bd. 

Pfam

 PF02970; TBCA 

SMART

  

PROSITE

  

PRINTS