UniProt Accession

 THOC5_HUMAN; Q13769; O60839; Q9UPZ5 

Genbank Protein ID

 L18972; AB023200; CR456542; AC005529; BC003615; AJ006069 

Genbank Nucleotide ID

 AAC26837.1; BAA76827.2; CAG30428.1; AAH03615.1; CAA06841.1 

Protein Name

 THO complex subunit 5 homolog 

Protein Synonyms/Alias

 Functional spliceosome-associated protein 79; fSAP79; NF2/meningioma region protein pK1.3; Placental protein 39.2; PP39.2; hTREX90 

Gene Name

 THOC5 

Gene Synonyms/Alias

 C22orf19; KIAA0983 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
222	ILKEIEVKKEYLSSL	ubiquitination	[1]
223	LKEIEVKKEYLSSLQ	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development. 

Sequence Annotation

 REGION 1 199 Interaction with THOC7.
 REGION 1 144 Interaction with CSF1R (By similarity).
 MOTIF 7 10 Nuclear localization signal (By
 MOD_RES 225 225 Phosphotyrosine.
 MOD_RES 307 307 Phosphoserine.
 MOD_RES 312 312 Phosphoserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 328 328 Phosphothreonine.  

Keyword

 Complete proteome; Cytoplasm; Differentiation; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 683 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0000445; C:THO complex part of transcription export complex; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IDA:UniProtKB.
 GO:0030224; P:monocyte differentiation; IDA:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0045650; P:negative regulation of macrophage differentiation; IEA:Compara.
 GO:0060215; P:primitive hemopoiesis; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR019163; THO_Thoc5. 

Pfam

 PF09766; FimP 

SMART

  

PROSITE

  

PRINTS