CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012276
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase alpha subunit B 
Protein Synonyms/Alias
 DNA polymerase alpha 70 kDa subunit 
Gene Name
 POLA2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72FEHEFLSKRLSKARHubiquitination[1, 2, 3]
134TPETPLTKRSVSTRSubiquitination[3, 4]
160PSATPSQKYNSRSNRubiquitination[1, 3, 5, 6]
194GAGNISLKVLGCPEAubiquitination[3, 4, 7]
207EALTGSYKSMFQKLPubiquitination[3, 7]
212SYKSMFQKLPDIREVubiquitination[1, 3, 5, 6, 7]
223IREVLTCKIEELGSEubiquitination[3]
232EELGSELKEHYKIEAubiquitination[3]
268SNGKLNNKSVILEGDubiquitination[1, 3]
403SPFEDIFKQCLRTIIubiquitination[3]
500DRFSRILKHILTQRSubiquitination[1, 3, 4, 5, 6]
549SELRYFVKDVLGCVCubiquitination[3]
564VNPGRLTKGQVGGTFubiquitination[1, 3, 4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). 
Sequence Annotation
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 127 127 Phosphothreonine.
 MOD_RES 130 130 Phosphothreonine.
 MOD_RES 141 141 Phosphoserine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 152 152 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 598 AA 
Protein Sequence
MSASAQQLAE ELQIFGLDCE EALIEKLVEL CVQYGQNEEG MVGELIAFCT STHKVGLTSE 60
ILNSFEHEFL SKRLSKARHS TCKDSGHAGA RDIVSIQELI EVEEEEEILL NSYTTPSKGS 120
QKRAISTPET PLTKRSVSTR SPHQLLSPSS FSPSATPSQK YNSRSNRGEV VTSFGLAQGV 180
SWSGRGGAGN ISLKVLGCPE ALTGSYKSMF QKLPDIREVL TCKIEELGSE LKEHYKIEAF 240
TPLLAPAQEP VTLLGQIGCD SNGKLNNKSV ILEGDREHSS GAQIPVDLSE LKEYSLFPGQ 300
VVIMEGINTT GRKLVATKLY EGVPLPFYQP TEEDADFEQS MVLVACGPYT TSDSITYDPL 360
LDLIAVINHD RPDVCILFGP FLDAKHEQVE NCLLTSPFED IFKQCLRTII EGTRSSGSHL 420
VFVPSLRDVH HEPVYPQPPF SYSDLSREDK KQVQFVSEPC SLSINGVIFG LTSTDLLFHL 480
GAEEISSSSG TSDRFSRILK HILTQRSYYP LYPPQEDMAI DYESFYVYAQ LPVTPDVLII 540
PSELRYFVKD VLGCVCVNPG RLTKGQVGGT FARLYLRRPA ADGAERQSPC IAVQVVRI 598 
Gene Ontology
 GO:0005658; C:alpha DNA polymerase:primase complex; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
 GO:0006270; P:DNA replication initiation; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000060; P:protein import into nucleus, translocation; IEA:Compara.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome. 
Interpro
 IPR007185; DNA_pol_alpha/epsilon_bsu.
 IPR016722; DNA_pol_alpha_bsu.
 IPR013627; Pol_alpha_B_N. 
Pfam
 PF04042; DNA_pol_E_B
 PF08418; Pol_alpha_B_N 
SMART
  
PROSITE
  
PRINTS