UniProt Accession

 PROF1_MOUSE; P62962; P10924 

Genbank Protein ID

 X14425; AK011066; BC002080 

Genbank Nucleotide ID

 CAA32586.1; BAB27373.1; AAH02080.1 

Protein Name

 Profilin-1 

Protein Synonyms/Alias

 Profilin I 

Gene Name

 Pfn1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
38	VWAAVPGKTFVSITP	acetylation	[1]
54	EVGVLVGKDRSSFFV	ubiquitination	[2]
70	GLTLGGQKCSVIRDS	ubiquitination	[2, 3]
116	TLVLLMGKEGVHGGL	ubiquitination	[2]
126	VHGGLINKKCYEMAS	acetylation	[4]
126	VHGGLINKKCYEMAS	ubiquitination	[2, 3]
127	HGGLINKKCYEMASH	ubiquitination	[2, 3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity). 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 108 108 N6-acetyllysine (By similarity).
 MOD_RES 129 129 Phosphotyrosine (By similarity).
 MOD_RES 138 138 Phosphoserine; by ROCK1 (By similarity).
 CROSSLNK 54 54 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 140 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IEA:Compara.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0071363; P:cellular response to growth factor stimulus; IEA:Compara.
 GO:0001843; P:neural tube closure; IGI:MGI.
 GO:0051054; P:positive regulation of DNA metabolic process; IEA:Compara.
 GO:0051496; P:positive regulation of stress fiber assembly; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0050434; P:positive regulation of viral transcription; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0042989; P:sequestering of actin monomers; TAS:MGI. 

Interpro

 IPR005454; Profilin_chordates.
 IPR027310; Profilin_CS.
 IPR005455; Profilin_eukaryotes/bac. 

Pfam

 PF00235; Profilin 

SMART

 SM00392; PROF 

PROSITE

 PS00414; PROFILIN 

PRINTS

 PR01639; PROFILINMAML.