CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016419
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-ketoglutarate-dependent dioxygenase FTO 
Protein Synonyms/Alias
 Fat mass and obesity-associated protein; Protein fatso 
Gene Name
 Fto 
Gene Synonyms/Alias
 Kiaa1752 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
88DVVRIQGKDVLTPVSubiquitination[1]
213PQKMPYLKEEPYFGMubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. 
Sequence Annotation
 REGION 42 324 Fe2OG dioxygenase domain (By similarity).
 REGION 210 221 Loop L1; predicted to block binding of
 REGION 228 231 Substrate binding (By similarity).
 REGION 313 315 Alpha-ketoglutarate binding (By
 METAL 228 228 Iron; catalytic (By similarity).
 METAL 230 230 Iron; catalytic (By similarity).
 METAL 304 304 Iron; catalytic (By similarity).
 BINDING 96 96 Substrate (By similarity).
 BINDING 108 108 Substrate (By similarity).
 BINDING 202 202 Alpha-ketoglutarate (By similarity).
 BINDING 292 292 Alpha-ketoglutarate (By similarity).
 BINDING 317 317 Alpha-ketoglutarate (By similarity).
 BINDING 319 319 Alpha-ketoglutarate (By similarity).
 MOD_RES 213 213 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Dioxygenase; DNA damage; DNA repair; Iron; Metal-binding; Nucleus; Obesity; Oxidoreductase; Reference proteome; RNA repair. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 502 AA 
Protein Sequence
MKRVQTAEER EREAKKLRLL EELEDTWLPY LTPKDDEFYQ QWQLKYPKLV FREAGSIPEE 60
LHKEVPEAFL TLHKHGCLFR DVVRIQGKDV LTPVSRILIG DPGCTYKYLN TRLFTVPWPV 120
KGCTVKYTEA EIAAACQTFL KLNDYLQVET IQALEELAVR EKANEDAVPL CMAEFPRAGV 180
GPSCDDEVDL KSRAAYNVTL LNFMDPQKMP YLKEEPYFGM GKMAVSWHHD ENLVDRSAVA 240
VYSYSCEGSE DESEDESSFE GRDPDTWHVG FKISWDIETP GLTIPLHQGD CYFMLDDLNA 300
THQHCVLAGS QPRFSSTHRV AECSTGTLDY ILERCQLALQ NVLNDSDDGD VSLKSFDPAV 360
LKQGEEIHNE VEFEWLRQFW FQGNRYKLCT DWWCEPMTHL EGLWKKMESM TNAVLREVKR 420
EGLPVEQRSE ILSAILVPLT VRQNLRKEWH ARCQSRVVRT LPVQQKPDCR PYWEKDDPSM 480
PLPFDLTDVV SELRGQLLEA RS 502 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043734; F:DNA-N1-methyladenine dioxygenase activity; IDA:UniProtKB.
 GO:0008198; F:ferrous iron binding; ISS:UniProtKB.
 GO:0035516; F:oxidative DNA demethylase activity; IDA:BHF-UCL.
 GO:0035515; F:oxidative RNA demethylase activity; IDA:BHF-UCL.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0060612; P:adipose tissue development; IMP:UniProtKB.
 GO:0006307; P:DNA dealkylation involved in DNA repair; ISS:UniProtKB.
 GO:0035552; P:oxidative single-stranded DNA demethylation; IDA:BHF-UCL.
 GO:0035553; P:oxidative single-stranded RNA demethylation; IDA:BHF-UCL.
 GO:0010883; P:regulation of lipid storage; IMP:UniProtKB.
 GO:0040014; P:regulation of multicellular organism growth; IMP:UniProtKB.
 GO:0044065; P:regulation of respiratory system process; IMP:UniProtKB.
 GO:0070350; P:regulation of white fat cell proliferation; IMP:UniProtKB.
 GO:0042245; P:RNA repair; IDA:BHF-UCL.
 GO:0001659; P:temperature homeostasis; IMP:UniProtKB. 
Interpro
 IPR024366; FTO_C.
 IPR024367; FTO_cat_dom. 
Pfam
 PF12934; FTO_CTD
 PF12933; FTO_NTD 
SMART
  
PROSITE
  
PRINTS