UniProt Accession

 DID2_YEAST; P69771; D6VX98 

Genbank Protein ID

 Z28260; BK006944 

Genbank Nucleotide ID

 DAA09188.1 

Protein Name

 Vacuolar protein-sorting-associated protein 46 

Protein Synonyms/Alias

 Charged multivesicular body protein 1; DOA4-independent degradation protein 2; Fifty two inhibitor 1 

Gene Name

 DID2 

Gene Synonyms/Alias

 CHM1; FTI1; VPS46; YKR035W-A 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
17	ENTLFQLKFTSKQLQ	ubiquitination	[1]
181	LDNVPEIKAKEVNVD	ubiquitination	[1]
191	EVNVDDEKEDKLAQR	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1. 

Sequence Annotation

 REGION 1 103 Interaction with VSP24.
 REGION 104 204 Interaction with VSP4.
 REGION 176 204 Interaction with VTA1.
 MOD_RES 5 5 Phosphoserine.  

Keyword

 3D-structure; Coiled coil; Complete proteome; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 204 AA 

Protein Sequence

Gene Ontology

 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005770; C:late endosome; IDA:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
 GO:0006623; P:protein targeting to vacuole; IMP:SGD. 

Interpro

 IPR005024; Snf7. 

Pfam

 PF03357; Snf7 

SMART

  

PROSITE

  

PRINTS