CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006690
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sterol regulatory element-binding protein 1 
Protein Synonyms/Alias
 SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1 
Gene Name
 SREBF1 
Gene Synonyms/Alias
 BHLHD1; SREBP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
123FSPGPGIKEESVPLSsumoylation[1, 2]
256SLLLTAMKTDGATVKubiquitination[3]
313NRLAAGSKAPASAQSubiquitination[3]
324SAQSRGEKRTAHNAIacetylation[4]
333TAHNAIEKRYRSSINacetylation[4]
347NDKIIELKDLVVGTEubiquitination[3]
356LVVGTEAKLNKSAVLubiquitination[3]
418DVLMEGVKTEVEDTLsumoylation[1]
470VFEDSKAKPEQRPSLubiquitination[3]
587VYFWRHRKQADLDLAubiquitination[3, 5]
675DAALVYHKLHQLHTMubiquitination[3]
924RAALHSFKAARALLGubiquitination[3, 5]
934RALLGCAKAESGPASubiquitination[5]
1070RRAGPGGKGGAVAELubiquitination[3]
1121EAARTLEKLGDRRLLubiquitination[3]
Reference
 [1] Sterol regulatory element-binding proteins are negatively regulated through SUMO-1 modification independent of the ubiquitin/26 S proteasome pathway.
 Hirano Y, Murata S, Tanaka K, Shimizu M, Sato R.
 J Biol Chem. 2003 May 9;278(19):16809-19. [PMID: 12615929]
 [2] Growth factor-induced phosphorylation of sterol regulatory element-binding proteins inhibits sumoylation, thereby stimulating the expression of their target genes, low density lipoprotein uptake, and lipid synthesis.
 Arito M, Horiba T, Hachimura S, Inoue J, Sato R.
 J Biol Chem. 2008 May 30;283(22):15224-31. [PMID: 18403372]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Coactivator-dependent acetylation stabilizes members of the SREBP family of transcription factors.
 Giandomenico V, Simonsson M, Grönroos E, Ericsson J.
 Mol Cell Biol. 2003 Apr;23(7):2587-99. [PMID: 12640139]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). 
Sequence Annotation
 DOMAIN 323 373 bHLH.
 REGION 1 60 Transcriptional activation (acidic).
 REGION 234 497 Interaction with LMNA (By similarity).
 REGION 373 394 Leucine-zipper.
 MOD_RES 110 110 Phosphotyrosine (By similarity).
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 337 337 Phosphoserine; by SIK1 (By similarity).
 MOD_RES 338 338 Phosphoserine; by SIK1 (By similarity).
 MOD_RES 396 396 Phosphoserine; by AMPK (By similarity).
 MOD_RES 402 402 Phosphoserine; by SIK1 (By similarity).  
Keyword
 3D-structure; Activator; Alternative splicing; Cholesterol metabolism; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; DNA-binding; Endoplasmic reticulum; Golgi apparatus; Lipid metabolism; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Steroid metabolism; Sterol metabolism; Transcription; Transcription regulation; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1147 AA 
Protein Sequence
MDEPPFSEAA LEQALGEPCD LDAALLTDIE DMLQLINNQD SDFPGLFDPP YAGSGAGGTD 60
PASPDTSSPG SLSPPPATLS SSLEAFLSGP QAAPSPLSPP QPAPTPLKMY PSMPAFSPGP 120
GIKEESVPLS ILQTPTPQPL PGALLPQSFP APAPPQFSST PVLGYPSPPG GFSTGSPPGN 180
TQQPLPGLPL ASPPGVPPVS LHTQVQSVVP QQLLTVTAAP TAAPVTTTVT SQIQQVPVLL 240
QPHFIKADSL LLTAMKTDGA TVKAAGLSPL VSGTTVQTGP LPTLVSGGTI LATVPLVVDA 300
EKLPINRLAA GSKAPASAQS RGEKRTAHNA IEKRYRSSIN DKIIELKDLV VGTEAKLNKS 360
AVLRKAIDYI RFLQHSNQKL KQENLSLRTA VHKSKSLKDL VSACGSGGNT DVLMEGVKTE 420
VEDTLTPPPS DAGSPFQSSP LSLGSRGSGS GGSGSDSEPD SPVFEDSKAK PEQRPSLHSR 480
GMLDRSRLAL CTLVFLCLSC NPLASLLGAR GLPSPSDTTS VYHSPGRNVL GTESRDGPGW 540
AQWLLPPVVW LLNGLLVLVS LVLLFVYGEP VTRPHSGPAV YFWRHRKQAD LDLARGDFAQ 600
AAQQLWLALR ALGRPLPTSH LDLACSLLWN LIRHLLQRLW VGRWLAGRAG GLQQDCALRV 660
DASASARDAA LVYHKLHQLH TMGKHTGGHL TATNLALSAL NLAECAGDAV SVATLAEIYV 720
AAALRVKTSL PRALHFLTRF FLSSARQACL AQSGSVPPAM QWLCHPVGHR FFVDGDWSVL 780
STPWESLYSL AGNPVDPLAQ VTQLFREHLL ERALNCVTQP NPSPGSADGD KEFSDALGYL 840
QLLNSCSDAA GAPAYSFSIS SSMATTTGVD PVAKWWASLT AVVIHWLRRD EEAAERLCPL 900
VEHLPRVLQE SERPLPRAAL HSFKAARALL GCAKAESGPA SLTICEKASG YLQDSLATTP 960
ASSSIDKAVQ LFLCDLLLVV RTSLWRQQQP PAPAPAAQGT SSRPQASALE LRGFQRDLSS 1020
LRRLAQSFRP AMRRVFLHEA TARLMAGASP TRTHQLLDRS LRRRAGPGGK GGAVAELEPR 1080
PTRREHAEAL LLASCYLPPG FLSAPGQRVG MLAEAARTLE KLGDRRLLHD CQQMLMRLGG 1140
GTTVTSS 1147 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005635; C:nuclear envelope; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:HGNC.
 GO:0032810; F:sterol response element binding; IDA:HGNC.
 GO:0007568; P:aging; IEA:Compara.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0009267; P:cellular response to starvation; ISS:HGNC.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; IEA:Compara.
 GO:0008610; P:lipid biosynthetic process; ISS:UniProtKB.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0046676; P:negative regulation of insulin secretion; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045542; P:positive regulation of cholesterol biosynthetic process; IEA:Compara.
 GO:0031065; P:positive regulation of histone deacetylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0010867; P:positive regulation of triglyceride biosynthetic process; ISS:UniProtKB.
 GO:0019217; P:regulation of fatty acid metabolic process; IEA:Compara.
 GO:0003062; P:regulation of heart rate by chemical signal; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0070542; P:response to fatty acid; IEA:Compara.
 GO:0032094; P:response to food; IEA:Compara.
 GO:0033762; P:response to glucagon stimulus; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0032570; P:response to progesterone stimulus; IEA:Compara.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0032933; P:SREBP signaling pathway; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS