CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041423
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 G2/M phase-specific E3 ubiquitin-protein ligase 
Protein Synonyms/Alias
  
Gene Name
 G2E3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116LKCCVCKKNGASIGCubiquitination[1]
196ILRSPCCKNAWFHRDubiquitination[1]
230NNSDIFQKEMLRMGIubiquitination[1]
282DYNAPDSKWEIKRCQubiquitination[1]
286PDSKWEIKRCQCCGSubiquitination[1]
327YNSGEFQKAKKHVLPubiquitination[1]
352LLEESSPKLPRQSPGubiquitination[1]
363QSPGSQSKDLLRQGSubiquitination[1, 2]
601KTLGVLEKIQAYPEAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Metal-binding; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 736 AA 
Protein Sequence
MLIPALQISD SESVGLEDGI EQSAYLTESK MNESKPGDSQ NLACVFCRKH DDCPNKYGEK 60
KTKEKWNLTV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVNRASKLK CCVCKKNGAS 120
IGCVAPRCKR SYHFPCGLQR ECIFQFTGNF ASFCWDHRPV QIITSNNYRE SLPCTICLEF 180
IEPIPSYNIL RSPCCKNAWF HRDCLQVQAI NAGVFFFRCT ICNNSDIFQK EMLRMGIHIP 240
EKDASWELEE NAYQELLQHY ERCDVRRCRC KEGRDYNAPD SKWEIKRCQC CGSSGTHLAC 300
SSLRSWEQNW ECLECRGIIY NSGEFQKAKK HVLPNSNNVG ITDCLLEESS PKLPRQSPGS 360
QSKDLLRQGS KFRRNVSTLL IELGFQIKKK TKRLYINKAN IWNSALDAFR NRNFNPSYAI 420
EVAYVIENDN FGSEHPGSKQ EFLSLLMQHL ENSSLFEGSL SKNLSLNSQA LKENLYYEAG 480
KMLAISLVHG GPSPGFFSKT LFNCLVYGPE NTQPILDDVS DFDVAQIIIR INTATTVADL 540
KSIINECYNY LELIGCLRLI TTLSDKYMLV KDILGYHVIQ RVHTPFESFK QGLKTLGVLE 600
KIQAYPEAFC SILCHKPESL SAKILSELFT VHTLPDVKAL GFWNSYLQAV EDGKSTTTME 660
DILIFATGCS SIPPAGFKPT PSIECLHVDF PVGNKCNNCL AIPITNTYKE FQENMDFTIR 720
NTLRLEKEES SHYIGH 736 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 
Interpro
 IPR000569; HECT.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00632; HECT 
SMART
 SM00249; PHD
 SM00184; RING 
PROSITE
 PS50237; HECT
 PS01359; ZF_PHD_1 
PRINTS