UniProt Accession

 TWF1_MOUSE; Q91YR1; O09132; Q52L77; Q80X09 

Genbank Protein ID

 U82324; AY267188; AK147990; AK150852; BC015081; BC094034 

Genbank Nucleotide ID

 AAB66592.1; AAP31404.1; BAE28272.1; BAE29908.1; AAH15081.1; AAH94034.1 

Protein Name

 Twinfilin-1 

Protein Synonyms/Alias

 Protein A6 

Gene Name

 Twf1 

Gene Synonyms/Alias

 Ptk9 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
136	VSLHGYKKYLLSQSS	acetylation	[1]
192	DAFQALEKLSKKQLN	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. 

Sequence Annotation

 DOMAIN 2 139 ADF-H 1.
 DOMAIN 175 313 ADF-H 2.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 309 309 Phosphotyrosine.
 MOD_RES 349 349 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 350 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IDA:UniProtKB.
 GO:0005911; C:cell-cell junction; IDA:BHF-UCL.
 GO:0030175; C:filopodium; IDA:BHF-UCL.
 GO:0030016; C:myofibril; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0003785; F:actin monomer binding; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:Compara.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:BHF-UCL.
 GO:0004713; F:protein tyrosine kinase activity; IDA:UniProtKB.
 GO:0051016; P:barbed-end actin filament capping; IDA:BHF-UCL.
 GO:0043538; P:regulation of actin phosphorylation; IEA:Compara.
 GO:0042989; P:sequestering of actin monomers; IDA:BHF-UCL. 

Interpro

 IPR002108; Actin-bd_cofilin/tropomyosin. 

Pfam

 PF00241; Cofilin_ADF 

SMART

 SM00102; ADF 

PROSITE

 PS51263; ADF_H 

PRINTS