CPLM-022192

Genbank Nucleotide ID

NEDD4 family-interacting protein 2

Protein Synonyms/Alias

NEDD4 WW domain-binding protein 5A; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF-kappa-B-activating protein 413

Homo sapiens (Human)

Position	Peptide	Type	References
87	GEDSLSRKPDPEPGR	ubiquitination	[1]
193	YDEAEKAKAAAMAAA	ubiquitination	[1, 2, 3, 4, 5, 6, 7]

[1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.

REGION 148 151 Interaction with NEDD4 (By similarity).
MOTIF 149 151 PY 1.
MOTIF 175 177 PY 2.
MOTIF 184 186 PY 3.
MOD_RES 151 151 Phosphotyrosine; by SRC; dependent on Y-
MOD_RES 167 167 Phosphotyrosine; by SRC.
MOD_RES 171 171 Phosphotyrosine; by SRC; dependent on Y-
MOD_RES 177 177 Phosphotyrosine; by SRC; dependent on Y-

Complete proteome; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005783; C:endoplasmic reticulum; IDA:BHF-UCL.
GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005739; C:mitochondrion; IDA:BHF-UCL.
GO:0032585; C:multivesicular body membrane; IEA:UniProtKB-SubCell.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0004871; F:signal transducer activity; IMP:UniProtKB.
GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
GO:0051224; P:negative regulation of protein transport; IMP:UniProtKB.
GO:0032410; P:negative regulation of transporter activity; IMP:UniProtKB.
GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
GO:0031398; P:positive regulation of protein ubiquitination; IMP:UniProtKB.

IPR019325; NEDD4/BSD2.