CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007998
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-dependent malic enzyme 
Protein Synonyms/Alias
 NADP-ME; Malic enzyme 1 
Gene Name
 ME1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26TRNPHLNKDLAFTLEubiquitination[1, 2, 3, 4]
60IQVLRVVKNFEHLNSacetylation[5]
60IQVLRVVKNFEHLNSubiquitination[1, 2, 3, 4]
84DLQDRNEKLFYRVLTubiquitination[3]
337KIWLVDSKGLIVKGRubiquitination[2, 3]
351RASLTQEKEKFAHEHubiquitination[2]
492IAQQVSDKHLEEGRLubiquitination[2]
512TIRDVSLKIAEKIVKubiquitination[2, 3]
525VKDAYQEKTATVYPEubiquitination[2, 3]
536VYPEPQNKEAFVRSQubiquitination[2, 3]
569EVQKIQTKVDQ****ubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 NP_BIND 301 318 NADP (By similarity).
 ACT_SITE 102 102 Proton donor (By similarity).
 ACT_SITE 173 173 Proton acceptor (By similarity).
 METAL 245 245 Divalent metal cation (By similarity).
 METAL 246 246 Divalent metal cation (By similarity).
 METAL 269 269 Divalent metal cation (By similarity).
 BINDING 155 155 NADP (By similarity).
 BINDING 269 269 NADP (By similarity).
 BINDING 408 408 NADP (By similarity).
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Metal-binding; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS QEIQVLRVVK 60
NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI VYTPTVGLAC QQYSLVFRKP 120
RGLFITIHDR GHIASVLNAW PEDVIKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC 180
GGMNPQECLP VILDVGTENE ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC 240
LIQFEDFANV NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ 300
GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE KEKFAHEHEE 360
MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER PIIFALSNPT SKAECSAEQC 420
YKITKGRAIF ASGSPFDPVT LPNGQTLYPG QGNNSYVFPG VALGVVACGL RQITDNIFLT 480
TAEVIAQQVS DKHLEEGRLY PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV 540
RSQMYSTDYD QILPDCYSWP EEVQKIQTKV DQ 572 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043531; F:ADP binding; TAS:UniProtKB.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; IDA:UniProtKB.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IDA:UniProtKB.
 GO:0051287; F:NAD binding; TAS:UniProtKB.
 GO:0050661; F:NADP binding; TAS:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; NAS:ProtInc.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0006108; P:malate metabolic process; IDA:UniProtKB.
 GO:0006741; P:NADP biosynthetic process; TAS:UniProtKB.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0009743; P:response to carbohydrate stimulus; TAS:UniProtKB.
 GO:0009725; P:response to hormone stimulus; ISS:UniProtKB. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.