UniProt Accession

 RAC1_RAT; Q6RUV5 

Genbank Protein ID

 AY491395 

Genbank Nucleotide ID

 AAR84574.1 

Protein Name

 Ras-related C3 botulinum toxin substrate 1 

Protein Synonyms/Alias

 p21-Rac1 

Gene Name

 Rac1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
123	KLDLRDDKDTIEKLK	ubiquitination	[1]
128	DDKDTIEKLKEKKLT	ubiquitination	[1]
133	IEKLKEKKLTPITYP	ubiquitination	[1]
147	PQGLAMAKEIGAVKY	acetylation	[2]
153	AKEIGAVKYLECSAL	acetylation	[2]
166	ALTQRGLKTVFDEAI	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (By similarity). Stimulates PKN2 kinase activity (By similarity). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. 

Sequence Annotation

 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 189 189 Cysteine methyl ester (By similarity).
 LIPID 189 189 S-geranylgeranyl cysteine (By
 CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; GTP-binding; Isopeptide bond; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 192 AA 

Protein Sequence

Gene Ontology

 GO:0031410; C:cytoplasmic vesicle; IDA:RGD.
 GO:0019897; C:extrinsic to plasma membrane; IEA:Compara.
 GO:0000139; C:Golgi membrane; IDA:RGD.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0001891; C:phagocytic cup; IEA:Compara.
 GO:0032587; C:ruffle membrane; IEA:Compara.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0005525; F:GTP binding; IDA:RGD.
 GO:0003924; F:GTPase activity; IDA:RGD.
 GO:0007015; P:actin filament organization; IMP:RGD.
 GO:0048532; P:anatomical structure arrangement; IEA:Compara.
 GO:0002093; P:auditory receptor cell morphogenesis; IEA:Compara.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0045453; P:bone resorption; IDA:RGD.
 GO:0008283; P:cell proliferation; IMP:RGD.
 GO:0045216; P:cell-cell junction organization; IEA:Compara.
 GO:0021799; P:cerebral cortex radially oriented cell migration; IEA:Compara.
 GO:0090103; P:cochlea morphogenesis; IEA:Compara.
 GO:0048813; P:dendrite morphogenesis; IEA:Compara.
 GO:0071542; P:dopaminergic neuron differentiation; IEA:Compara.
 GO:0021831; P:embryonic olfactory bulb interneuron precursor migration; IEA:Compara.
 GO:0043652; P:engulfment of apoptotic cell; IEA:Compara.
 GO:0003382; P:epithelial cell morphogenesis; IEA:Compara.
 GO:0006972; P:hyperosmotic response; IEA:Compara.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0051668; P:localization within membrane; IEA:Compara.
 GO:0002551; P:mast cell chemotaxis; IMP:RGD.
 GO:0032707; P:negative regulation of interleukin-23 production; IEA:Compara.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:0045740; P:positive regulation of DNA replication; IMP:RGD.
 GO:0010592; P:positive regulation of lamellipodium assembly; IEA:Compara.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
 GO:0016601; P:Rac protein signal transduction; TAS:RGD.
 GO:0030334; P:regulation of cell migration; ISS:UniProtKB.
 GO:0060263; P:regulation of respiratory burst; IEA:Compara.
 GO:0097178; P:ruffle assembly; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara.
 GO:0060071; P:Wnt receptor signaling pathway, planar cell polarity pathway; IEA:Compara. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 

Pfam

 PF00071; Ras 

SMART

 SM00174; RHO 

PROSITE

 PS51420; RHO 

PRINTS

 PR00449; RASTRNSFRMNG.