UniProt Accession

 G6PI_RAT; Q6P6V0 

Genbank Protein ID

 BC062005 

Genbank Nucleotide ID

 AAH62005.1 

Protein Name

 Glucose-6-phosphate isomerase 

Protein Synonyms/Alias

 GPI; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI 

Gene Name

 Gpi 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
12	TRNPEFQKLLEWHRA	acetylation	[1]
25	RANSANLKLRELFEA	acetylation	[1]
62	YSKNLVNKEVLHMLV	acetylation	[1]
73	HMLVDLAKSRGVEAA	acetylation	[1]
89	DNMFSGLKINSTEDR	acetylation	[1]
116	RSIMMDGKDVMPEVN	acetylation	[1]
124	DVMPEVNKVLDKMKS	acetylation	[1]
128	EVNKVLDKMKSFCQR	acetylation	[1]
142	RVRSGDWKGYTGKAI	acetylation	[1]
226	ITNAETAKEWFLQAA	acetylation	[1]
234	EWFLQAAKDPSAVAK	acetylation	[1]
234	EWFLQAAKDPSAVAK	ubiquitination	[2]
241	KDPSAVAKHFVALST	acetylation	[1]
252	ALSTNTDKVKEFGID	acetylation	[1]
254	STNTDKVKEFGIDPK	acetylation	[1]
362	GDMESNGKYITKSGA	acetylation	[1]
438	AQTEALMKGKSPEEA	acetylation	[1]
447	KSPEEARKELQAAGK	ubiquitination	[2]
454	KELQAAGKSPEELEK	acetylation	[1]
461	KSPEELEKLLPHKVF	acetylation	[1]
466	LEKLLPHKVFEGNRP	acetylation	[1]
466	LEKLLPHKVFEGNRP	ubiquitination	[2]
524	LGKQLAKKIEPELDG	acetylation	[1]
550	NGLIGFIKLQRDTKI	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons (By similarity). 

Sequence Annotation

 ACT_SITE 358 358 Proton donor (By similarity).
 ACT_SITE 389 389 By similarity.
 ACT_SITE 519 519 By similarity.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 12 12 N6-acetyllysine (By similarity).
 MOD_RES 142 142 N6-acetyllysine (By similarity).
 MOD_RES 185 185 Phosphoserine; by CK2 (By similarity).
 MOD_RES 454 454 N6-malonyllysine (By similarity).  

Keyword

 Acetylation; Angiogenesis; Complete proteome; Cytokine; Cytoplasm; Gluconeogenesis; Glycolysis; Growth factor; Isomerase; Phosphoprotein; Reference proteome; Secreted; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 558 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0004347; F:glucose-6-phosphate isomerase activity; IDA:RGD.
 GO:0016866; F:intramolecular transferase activity; IMP:RGD.
 GO:0048029; F:monosaccharide binding; IPI:RGD.
 GO:0046185; P:aldehyde catabolic process; IMP:RGD.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; IDA:RGD.
 GO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW.
 GO:0051156; P:glucose 6-phosphate metabolic process; IDA:RGD.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
 GO:0007611; P:learning or memory; IEP:RGD.
 GO:0019242; P:methylglyoxal biosynthetic process; IMP:RGD.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:RGD.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:RGD. 

Interpro

 IPR001672; G6P_Isomerase.
 IPR023096; G6P_Isomerase_C.
 IPR018189; Phosphoglucose_isomerase_CS. 

Pfam

 PF00342; PGI 

SMART

  

PROSITE

 PS00765; P_GLUCOSE_ISOMERASE_1
 PS00174; P_GLUCOSE_ISOMERASE_2
 PS51463; P_GLUCOSE_ISOMERASE_3 

PRINTS

 PR00662; G6PISOMERASE.