CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019608
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tribbles homolog 3 
Protein Synonyms/Alias
 TRB-3; Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B 
Gene Name
 TRIB3 
Gene Synonyms/Alias
 C20orf97; NIPK; SKIP3; TRB3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33DTERPVQKRARSGPQubiquitination[1]
97TGTEYTCKVYPVQEAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA. 
Sequence Annotation
 DOMAIN 68 316 Protein kinase.
 REGION 1 127 Interaction with DDIT3/CHOP.  
Keyword
 Apoptosis; Complete proteome; Nucleus; Polymorphism; Protein kinase inhibitor; Reference proteome; Stress response; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 358 AA 
Protein Sequence
MRATPLAAPA GSLSRKKRLE LDDNLDTERP VQKRARSGPQ PRLPPCLLPL SPPTAPDRAT 60
AVATASRLGP YVLLEPEEGG RAYQALHCPT GTEYTCKVYP VQEALAVLEP YARLPPHKHV 120
ARPTEVLAGT QLLYAFFTRT HGDMHSLVRS RHRIPEPEAA VLFRQMATAL AHCHQHGLVL 180
RDLKLCRFVF ADRERKKLVL ENLEDSCVLT GPDDSLWDKH ACPAYVGPEI LSSRASYSGK 240
AADVWSLGVA LFTMLAGHYP FQDSEPVLLF GKIRRGAYAL PAGLSAPARC LVRCLLRREP 300
AERLTATGIL LHPWLRQDPM PLAPTRSHLW EAAQVVPDGL GLDEAREEEG DREVVLYG 358 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0019901; F:protein kinase binding; ISS:UniProtKB.
 GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
 GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IEA:InterPro.
 GO:0031625; F:ubiquitin protein ligase binding; ISS:BHF-UCL.
 GO:0055106; F:ubiquitin-protein ligase regulator activity; ISS:BHF-UCL.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IDA:UniProtKB.
 GO:0045599; P:negative regulation of fat cell differentiation; ISS:BHF-UCL.
 GO:0045717; P:negative regulation of fatty acid biosynthetic process; ISS:BHF-UCL.
 GO:0006469; P:negative regulation of protein kinase activity; IDA:BHF-UCL.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0032092; P:positive regulation of protein binding; ISS:BHF-UCL.
 GO:0051443; P:positive regulation of ubiquitin-protein ligase activity; ISS:BHF-UCL.
 GO:0006468; P:protein phosphorylation; IEA:InterPro.
 GO:0010827; P:regulation of glucose transport; ISS:BHF-UCL.
 GO:0043405; P:regulation of MAP kinase activity; IDA:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR024104; Tribbles/Ser_Thr_kinase_40.
 IPR024106; Tribbles_TRB3. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM 
PRINTS