UniProt Accession

 RFAH_ECOLI; P0AFW0; P26614; Q2M8E5 

Genbank Protein ID

 X65013; M87049; M94889; U00096; AP009048 

Genbank Nucleotide ID

 CAA46147.1; AAA67638.1; AAA91060.1; AAC76845.1; BAE77461.1 

Protein Name

 Transcription antitermination protein RfaH 

Protein Synonyms/Alias

  

Gene Name

 rfaH 

Gene Synonyms/Alias

 hlyT; sfrB; b3842; JW3818 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
100	IHQLSVYKPKDIVDP	acetylation	[1]
102	QLSVYKPKDIVDPAT	acetylation	[1]
148	LLLNLINKEIKHSVK	acetylation	[1]
151	NLINKEIKHSVKNTE	acetylation	[1]
155	KEIKHSVKNTEFRKL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Enhances distal genes transcription elongation in a specialized subset of operons that encode extracytoplasmic components. RfaH is recruited into a multi-component RNA polymerase complex by the ops element, which is a short conserved DNA sequence located downstream of the main promoter of these operons. Once bound, RfaH suppresses pausing and inhibits Rho- dependent and intrinsic termination at a subset of sites. Termination signals are bypassed, which allows complete synthesis of long RNA chains. Enhances expression of several operons involved in synthesis of lipopolysaccharides, exopolysaccharides, hemolysin, and sex factor. Also negatively controls expression and surface presentation of AG43 and possibly another AG43-independent factor that mediates cell-cell interactions and biofilm formation. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; DNA-binding; Reference proteome; Transcription; Transcription antitermination; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 162 AA 

Protein Sequence

Gene Ontology

 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0001073; F:DNA binding transcription antitermination factor activity; IDA:EcoCyc.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR005824; KOW.
 IPR010215; Transcription_activator_RfaH.
 IPR006645; Transcrpt_antiterm_NusG_N. 

Pfam

 PF02357; NusG 

SMART

 SM00739; KOW
 SM00738; NGN 

PROSITE

  

PRINTS