CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022135
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-mannosidase 2C1 
Protein Synonyms/Alias
 Alpha mannosidase 6A8B; Alpha-D-mannoside mannohydrolase; Mannosidase alpha class 2C member 1 
Gene Name
 MAN2C1 
Gene Synonyms/Alias
 MANA; MANA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAAAPALKHWRTTLEubiquitination[1]
19TTLERVEKFVSPLYFubiquitination[1]
125GLTKEGEKTSYVLTDubiquitination[1]
750DYHLETRKPVLGQAGubiquitination[1, 2, 3, 4]
809VHWHEAHKFLKVEFPubiquitination[1]
812HEAHKFLKVEFPARVubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 ACT_SITE 372 372 Nucleophile (By similarity).
 METAL 260 260 Zinc (By similarity).
 METAL 262 262 Zinc (By similarity).
 METAL 372 372 Zinc (By similarity).
 METAL 577 577 Zinc (By similarity).
 MOD_RES 480 480 Phosphoserine (By similarity).
 MOD_RES 482 482 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Glycosidase; Hydrolase; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1040 AA 
Protein Sequence
MAAAPALKHW RTTLERVEKF VSPLYFTDCN LRGRLFGASC PVAVLSSFLT PERLPYQEAV 60
QRDFRPAQVG DSFGPTWWTC WFRVELTIPE AWVGQEVHLC WESDGEGLVW RDGEPVQGLT 120
KEGEKTSYVL TDRLGERDPR SLTLYVEVAC NGLLGAGKGS MIAAPDPEKM FQLSRAELAV 180
FHRDVHMLLV DLELLLGIAK GLGKDNQRSF QALYTANQMV NVCDPAQPET FPVAQALASR 240
FFGQHGGESQ HTIHATGHCH IDTAWLWPFK ETVRKCARSW VTALQLMERN PEFIFACSQA 300
QQLEWVKSRY PGLYSRIQEF ACRGQFVPVG GTWVEMDGNL PSGEAMVRQF LQGQNFFLQE 360
FGKMCSEFWL PDTFGYSAQL PQIMHGCGIR RFLTQKLSWN LVNSFPHHTF FWEGLDGSRV 420
LVHFPPGDSY GMQGSVEEVL KTVANNRDKG RANHSAFLFG FGDGGGGPTQ TMLDRLKRLS 480
NTDGLPRVQL SSPRQLFSAL ESDSEQLCTW VGELFLELHN GTYTTHAQIK KGNRECERIL 540
HDVELLSSLA LARSAQFLYP AAQLQHLWRL LLLNQFHDVV TGSCIQMVAE EAMCHYEDIR 600
SHGNTLLSAA AAALCAGEPG PEGLLIVNTL PWKRIEVMAL PKPGGAHSLA LVTVPSMGYA 660
PVPPPTSLQP LLPQQPVFVV QETDGSVTLD NGIIRVKLDP TGRLTSLVLV ASGREAIAEG 720
AVGNQFVLFD DVPLYWDAWD VMDYHLETRK PVLGQAGTLA VGTEGGLRGS AWFLLQISPN 780
SRLSQEVVLD VGCPYVRFHT EVHWHEAHKF LKVEFPARVR SSQATYEIQF GHLQRPTHYN 840
TSWDWARFEV WAHRWMDLSE HGFGLALLND CKYGASVRGS ILSLSLLRAP KAPDATADTG 900
RHEFTYALMP HKGSFQDAGV IQAAYSLNFP LLALPAPSPA PATSWSAFSV SSPAVVLETV 960
KQAESSPQRR SLVLRLYEAH GSHVDCWLHL SLPVQEAILC DLLERPDPAG HLTLRDNRLK 1020
LTFSPFQVLS LLLVLQPPPH 1040 
Gene Ontology
 GO:0004559; F:alpha-mannosidase activity; IEA:EC.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006013; P:mannose metabolic process; IEA:InterPro. 
Interpro
 IPR011013; Gal_mutarotase_SF_dom.
 IPR011330; Glyco_hydro/deAcase_b/a-brl.
 IPR027291; Glyco_hydro_38/57_N.
 IPR011682; Glyco_hydro_38_C.
 IPR015341; Glyco_hydro_38_cen_dom.
 IPR000602; Glyco_hydro_38_N. 
Pfam
 PF09261; Alpha-mann_mid
 PF01074; Glyco_hydro_38
 PF07748; Glyco_hydro_38C 
SMART
 SM00872; Alpha-mann_mid 
PROSITE
  
PRINTS