UniProt Accession

 PSIP1_MOUSE; Q99JF8; A2BI10; A2BI11; Q3TEJ7; Q3TTD7; Q3UTA1; Q80WQ7; Q99JF7; Q99LR4; Q9CT03 

Genbank Protein ID

 AJ308965; AJ308966; AF339082; AF339083; BX682545; BX682545; BC002260; BC043079; BC052177; AK011572; AK139598; AK161426; AK169600 

Genbank Nucleotide ID

 CAC34944.1; CAC34945.1; AAO32949.1; AAO32950.1; CAM20466.1; CAM20467.1; AAH02260.1; AAH43079.1; AAH52177.1; BAB27707.3; BAE24079.1; BAE36388.1; BAE41251.1 

Protein Name

 PC4 and SFRS1-interacting protein 

Protein Synonyms/Alias

 Lens epithelium-derived growth factor; mLEDGF 

Gene Name

 Psip1 

Gene Synonyms/Alias

 Ledgf 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
65	IFPYSENKEKYGKPN	ubiquitination	[1]
413	VSQVIMEKSTMLYNK	acetylation	[2]
420	KSTMLYNKFKNMFLV	acetylation	[3]
465	GKKGPNKKLEKEPTG	acetylation	[3]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis (By similarity). 

Sequence Annotation

 DOMAIN 7 64 PWWP.
 MOTIF 146 156 Nuclear localization signal (By
 MOD_RES 102 102 Phosphoserine (By similarity).
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 122 122 Phosphothreonine (By similarity).
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 134 134 Phosphothreonine.
 MOD_RES 141 141 Phosphothreonine.
 MOD_RES 176 176 Phosphoserine.
 MOD_RES 205 205 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 274 274 Phosphoserine.
 MOD_RES 432 432 Phosphoserine (By similarity).
 MOD_RES 435 435 Phosphothreonine (By similarity).
 MOD_RES 441 441 Phosphoserine (By similarity).
 MOD_RES 520 520 Phosphoserine (By similarity).
 MOD_RES 525 525 Phosphothreonine (By similarity).  

Keyword

 Alternative splicing; Coiled coil; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 528 AA 

Protein Sequence

Gene Ontology

 GO:0005720; C:nuclear heterochromatin; ISS:UniProtKB.
 GO:0034399; C:nuclear periphery; IEA:Compara.
 GO:0005654; C:nucleoplasm; ISS:UniProtKB.
 GO:0035327; C:transcriptionally active chromatin; ISS:UniProtKB.
 GO:0033613; F:activating transcription factor binding; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IEA:Compara.
 GO:0097100; F:supercoiled DNA binding; ISS:UniProtKB.
 GO:0000395; P:mRNA 5'-splice site recognition; ISS:UniProtKB.
 GO:0009408; P:response to heat; IDA:UniProtKB.
 GO:0006979; P:response to oxidative stress; IDA:UniProtKB. 

Interpro

 IPR021567; LEDGF.
 IPR000313; PWWP.
 IPR017859; Treacle-like_TCS. 

Pfam

 PF11467; LEDGF
 PF00855; PWWP 

SMART

 SM00293; PWWP 

PROSITE

 PS50812; PWWP 

PRINTS

 PR01503; TREACLE.