CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011955
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin-3 
Protein Synonyms/Alias
 ANK-3; Ankyrin-G 
Gene Name
 ANK3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
3472EVIEEEGKVGPDEDKacetylation[1]
4338RTSPADGKPRLSLHEubiquitination[2, 3]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. 
Sequence Annotation
 REPEAT 73 102 ANK 1.
 REPEAT 106 135 ANK 2.
 REPEAT 139 168 ANK 3.
 REPEAT 172 201 ANK 4.
 REPEAT 203 230 ANK 5.
 REPEAT 234 263 ANK 6.
 REPEAT 267 296 ANK 7.
 REPEAT 300 329 ANK 8.
 REPEAT 333 362 ANK 9.
 REPEAT 366 395 ANK 10.
 REPEAT 399 428 ANK 11.
 REPEAT 432 461 ANK 12.
 REPEAT 465 494 ANK 13.
 REPEAT 498 527 ANK 14.
 REPEAT 531 560 ANK 15.
 REPEAT 564 593 ANK 16.
 REPEAT 597 626 ANK 17.
 REPEAT 630 659 ANK 18.
 REPEAT 663 692 ANK 19.
 REPEAT 696 725 ANK 20.
 REPEAT 729 758 ANK 21.
 REPEAT 762 791 ANK 22.
 REPEAT 795 825 ANK 23.
 DOMAIN 982 1107 ZU5 1.
 DOMAIN 1108 1272 ZU5 2.
 DOMAIN 4090 4174 Death.
 REGION 1273 1407 UPA domain (By similarity).
 MOD_RES 39 39 Phosphoserine.
 MOD_RES 847 847 Phosphoserine.
 MOD_RES 1445 1445 Phosphoserine.
 MOD_RES 4298 4298 Phosphoserine.
 CROSSLNK 4338 4338 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ANK repeat; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Isopeptide bond; Lysosome; Membrane; Phosphoprotein; Polymorphism; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4377 AA 
Protein Sequence
MAHAASQLKK NRDLEINAEE EPEKKRKHRK RSRDRKKKSD ANASYLRAAR AGHLEKALDY 60
IKNGVDINIC NQNGLNALHL ASKEGHVEVV SELLQREANV DAATKKGNTA LHIASLAGQA 120
EVVKVLVTNG ANVNAQSQNG FTPLYMAAQE NHLEVVKFLL DNGASQSLAT EDGFTPLAVA 180
LQQGHDQVVS LLLENDTKGK VRLPALHIAA RKDDTKAAAL LLQNDNNADV ESKSGFTPLH 240
IAAHYGNINV ATLLLNRAAA VDFTARNDIT PLHVASKRGN ANMVKLLLDR GAKIDAKTRD 300
GLTPLHCGAR SGHEQVVEML LDRAAPILSK TKNGLSPLHM ATQGDHLNCV QLLLQHNVPV 360
DDVTNDYLTA LHVAAHCGHY KVAKVLLDKK ANPNAKALNG FTPLHIACKK NRIKVMELLL 420
KHGASIQAVT ESGLTPIHVA AFMGHVNIVS QLMHHGASPN TTNVRGETAL HMAARSGQAE 480
VVRYLVQDGA QVEAKAKDDQ TPLHISARLG KADIVQQLLQ QGASPNAATT SGYTPLHLSA 540
REGHEDVAAF LLDHGASLSI TTKKGFTPLH VAAKYGKLEV ANLLLQKSAS PDAAGKSGLT 600
PLHVAAHYDN QKVALLLLDQ GASPHAAAKN GYTPLHIAAK KNQMDIATTL LEYGADANAV 660
TRQGIASVHL AAQEGHVDMV SLLLGRNANV NLSNKSGLTP LHLAAQEDRV NVAEVLVNQG 720
AHVDAQTKMG YTPLHVGCHY GNIKIVNFLL QHSAKVNAKT KNGYTPLHQA AQQGHTHIIN 780
VLLQNNASPN ELTVNGNTAL GIARRLGYIS VVDTLKIVTE ETMTTTTVTE KHKMNVPETM 840
NEVLDMSDDE VRKANAPEML SDGEYISDVE EGEDAMTGDT DKYLGPQDLK ELGDDSLPAE 900
GYMGFSLGAR SASLRSFSSD RSYTLNRSSY ARDSMMIEEL LVPSKEQHLT FTREFDSDSL 960
RHYSWAADTL DNVNLVSSPI HSGFLVSFMV DARGGSMRGS RHHGMRIIIP PRKCTAPTRI 1020
TCRLVKRHKL ANPPPMVEGE GLASRLVEMG PAGAQFLGPV IVEIPHFGSM RGKERELIVL 1080
RSENGETWKE HQFDSKNEDL TELLNGMDEE LDSPEELGKK RICRIITKDF PQYFAVVSRI 1140
KQESNQIGPE GGILSSTTVP LVQASFPEGA LTKRIRVGLQ AQPVPDEIVK KILGNKATFS 1200
PIVTVEPRRR KFHKPITMTI PVPPPSGEGV SNGYKGDTTP NLRLLCSITG GTSPAQWEDI 1260
TGTTPLTFIK DCVSFTTNVS ARFWLADCHQ VLETVGLATQ LYRELICVPY MAKFVVFAKM 1320
NDPVESSLRC FCMTDDKVDK TLEQQENFEE VARSKDIEVL EGKPIYVDCY GNLAPLTKGG 1380
QQLVFNFYSF KENRLPFSIK IRDTSQEPCG RLSFLKEPKT TKGLPQTAVC NLNITLPAHK 1440
KETESDQDDE IEKTDRRQSF ASLALRKRYS YLTEPGMIER STGATRSLPT TYSYKPFFST 1500
RPYQSWTTAP ITVPGPAKSG FTSLSSSSSN TPSASPLKSI WSVSTPSPIK STLGASTTSS 1560
VKSISDVASP IRSFRTMSSP IKTVVSQSPY NIQVSSGTLA RAPAVTEATP LKGLASNSTF 1620
SSRTSPVTTA GSLLERSSIT MTPPASPKSN INMYSSSLPF KSIITSAAPL ISSPLKSVVS 1680
PVKSAVDVIS SAKITMASSL SSPVKQMPGH AEVALVNGSI SPLKYPSSST LINGCKATAT 1740
LQEKISSATN SVSSVVSAAT DTVEKVFSTT TAMPFSPLRS YVSAAPSAFQ SLRTPSASAL 1800
YTSLGSSISA TTSSVTSSII TVPVYSVVNV LPEPALKKLP DSNSFTKSAA ALLSPIKTLT 1860
TETHPQPHFS RTSSPVKSSL FLAPSALKLS TPSSLSSSQE ILKDVAEMKE DLMRMTAILQ 1920
TDVPEEKPFQ PELPKEGRID DEEPFKIVEK VKEDLVKVSE ILKKDVCVDN KGSPKSPKSD 1980
KGHSPEDDWI EFSSEEIREA RQQAAASQSP SLPERVQVKA KAASEKDYNL TKVIDYLTND 2040
IGSSSLTNLK YKFEDAKKDG EERQKRVLKP AIALQEHKLK MPPASMRTST SEKELCKMAD 2100
SFFGTDTILE SPDDFSQHDQ DKSPLSDSGF ETRSEKTPSA PQSAESTGPK PLFHEVPIPP 2160
VITETRTEVV HVIRSYDPSA GDVPQTQPEE PVSPKPSPTF MELEPKPTTS SIKEKVKAFQ 2220
MKASSEEDDH NRVLSKGMRV KEETHITTTT RMVYHSPPGG EGASERIEET MSVHDIMKAF 2280
QSGRDPSKEL AGLFEHKSAV SPDVHKSAAE TSAQHAEKDN QMKPKLERII EVHIEKGNQA 2340
EPTEVIIRET KKHPEKEMYV YQKDLSRGDI NLKDFLPEKH DAFPCSEEQG QQEEEELTAE 2400
ESLPSYLESS RVNTPVSQEE DSRPSSAQLI SDDSYKTLKL LSQHSIEYHD DELSELRGES 2460
YRFAEKMLLS EKLDVSHSDT EESVTDHAGP PSSELQGSDK RSREKIATAP KKEILSKIYK 2520
DVSENGVGKV SKDEHFDKVT VLHYSGNVSS PKHAMWMRFT EDRLDRGREK LIYEDRVDRT 2580
VKEAEEKLTE VSQFFRDKTE KLNDELQSPE KKARPKNGKE YSSQSPTSSS PEKVLLTELL 2640
ASNDEWVKAR QHGPDGQGFP KAEEKAPSLP SSPEKMVLSQ QTEDSKSTVE AKGSISQSKA 2700
PDGPQSGFQL KQSKLSSIRL KFEQGTHAKS KDMSQEDRKS DGQSRIPVKK IQESKLPVYQ 2760
VFAREKQQKA IDLPDESVSV QKDFMVLKTK DEHAQSNEIV VNDSGSDNVK KQRTEMSSKA 2820
MPDSFSEQQA KDLACHITSD LATRGPWDKK VFRTWESSGA TNNKSQKEKL SHVLVHDVRE 2880
NHIGHPESKS VDQKNEFMSV TERERKLLTN GSLSEIKEMT VKSPSKKVLY REYVVKEGDH 2940
PGGLLDQPSR RSESSAVSHI PVRVADERRM LSSNIPDGFC EQSAFPKHEL SQKLSQSSMS 3000
KETVETQHFN SIEDEKVTYS EISKVSKHQS YVGLCPPLEE TETSPTKSPD SLEFSPGKES 3060
PSSDVFDHSP IDGLEKLAPL AQTEGGKEIK TLPVYVSFVQ VGKQYEKEIQ QGGVKKIISQ 3120
ECKTVQETRG TFYTTRQQKQ PPSPQGSPED DTLEQVSFLD SSGKSPLTPE TPSSEEVSYE 3180
FTSKTPDSLI AYIPGKPSPI PEVSEESEEE EQAKSTSLKQ TTVEETAVER EMPNDVSKDS 3240
NQRPKNNRVA YIEFPPPPPL DADQIESDKK HHYLPEKEVD MIEVNLQDEH DKYQLAEPVI 3300
RVQPPSPVPP GADVSDSSDD ESIYQPVPVK KYTFKLKEVD DEQKEKPKAS AEKASNQKEL 3360
ESNGSGKDNE FGLGLDSPQN EIAQNGNNDQ SITECSIATT AEFSHDTDAT EIDSLDGYDL 3420
QDEDDGLTES DSKLPIQAME IKKDIWNTEG ILKPADRSFS QSKLEVIEEE GKVGPDEDKP 3480
PSKSSSSEKT PDKTDQKSGA QFFTLEGRHP DRSVFPDTYF SYKVDEEFAT PFKTVATKGL 3540
DFDPWSNNRG DDEVFDSKSR EDETKPFGLA VEDRSPATTP DTTPARTPTD ESTPTSEPNP 3600
FPFHEGKMFE MTRSGAIDMS KRDFVEERLQ FFQIGEHTSE GKSGDQGEGD KSMVTATPQP 3660
QSGDTTVETN LERNVETPTV EPNPSIPTSG ECQEGTSSSG SLEKSAAATN TSKVDPKLRT 3720
PIKMGISAST MTMKKEGPGE ITDKIEAVMT SCQGLENETI TMISNTANSQ MGVRPHEKHD 3780
FQKDNFNNNN NLDSSTIQTD NIMSNIVLTE HSAPTCTTEK DNPVKVSSGK KTGVLQGHCV 3840
RDKQKVLGEQ QKTKELIGIR QKSKLPIKAT SPKDTFPPNH MSNTKASKMK QVSQSEKTKA 3900
LTTSSCVDVK SRIPVKNTHR DNIIAVRKAC ATQKQGQPEK GKAKQLPSKL PVKVRSTCVT 3960
TTTTTATTTT TTTTTTTTSC TVKVRKSQLK EVCKHSIEYF KGISGETLKL VDRLSEEEKK 4020
MQSELSDEEE STSRNTSLSE TSRGGQPSVT TKSARDKKTE AAPLKSKSEK AGSEKRSSRR 4080
TGPQSPCERT DIRMAIVADH LGLSWTELAR ELNFSVDEIN QIRVENPNSL ISQSFMLLKK 4140
WVTRDGKNAT TDALTSVLTK INRIDIVTLL EGPIFDYGNI SGTRSFADEN NVFHDPVDGW 4200
QNETSSGNLE SCAQARRVTG GLLDRLDDSP DQCRDSITSY LKGEAGKFEA NGSHTEITPE 4260
AKTKSYFPES QNDVGKQSTK ETLKPKIHGS GHVEEPASPL AAYQKSLEET SKLIIEETKP 4320
CVPVSMKKMS RTSPADGKPR LSLHEEEGSS GSEQKQGEGF KVKTKKEIRH VEKKSHS 4377 
Gene Ontology
 GO:0043194; C:axon initial segment; ISS:BHF-UCL.
 GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
 GO:0009986; C:cell surface; ISS:BHF-UCL.
 GO:0043034; C:costamere; TAS:BHF-UCL.
 GO:0014704; C:intercalated disc; ISS:BHF-UCL.
 GO:0016328; C:lateral plasma membrane; IDA:BHF-UCL.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0033268; C:node of Ranvier; ISS:BHF-UCL.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0014731; C:spectrin-associated cytoskeleton; ISS:BHF-UCL.
 GO:0030315; C:T-tubule; ISS:BHF-UCL.
 GO:0045296; F:cadherin binding; ISS:BHF-UCL.
 GO:0044325; F:ion channel binding; ISS:BHF-UCL.
 GO:0030674; F:protein binding, bridging; ISS:BHF-UCL.
 GO:0030507; F:spectrin binding; ISS:BHF-UCL.
 GO:0005200; F:structural constituent of cytoskeleton; IMP:BHF-UCL.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0007409; P:axonogenesis; ISS:BHF-UCL.
 GO:0043001; P:Golgi to plasma membrane protein transport; IMP:BHF-UCL.
 GO:0072660; P:maintenance of protein location in plasma membrane; IGI:BHF-UCL.
 GO:0071709; P:membrane assembly; IMP:BHF-UCL.
 GO:0000281; P:mitotic cytokinesis; IMP:BHF-UCL.
 GO:0010628; P:positive regulation of gene expression; ISS:BHF-UCL.
 GO:1900827; P:positive regulation of membrane depolarization involved in regulation of cardiac muscle cell action potential; ISS:BHF-UCL.
 GO:0045838; P:positive regulation of membrane potential; ISS:BHF-UCL.
 GO:2000651; P:positive regulation of sodium ion transmembrane transporter activity; ISS:BHF-UCL.
 GO:0010765; P:positive regulation of sodium ion transport; ISS:BHF-UCL.
 GO:0072661; P:protein targeting to plasma membrane; IMP:BHF-UCL.
 GO:0019228; P:regulation of action potential in neuron; ISS:BHF-UCL.
 GO:0043266; P:regulation of potassium ion transport; ISS:BHF-UCL.
 GO:0007165; P:signal transduction; IEA:InterPro.
 GO:0050808; P:synapse organization; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011029; DEATH-like_dom.
 IPR000488; Death_domain.
 IPR000906; ZU5. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF00531; Death
 PF00791; ZU5 
SMART
 SM00248; ANK
 SM00005; DEATH
 SM00218; ZU5 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50017; DEATH_DOMAIN
 PS51145; ZU5 
PRINTS
 PR01415; ANKYRIN.