UniProt Accession

 MED20_HUMAN; Q9H944; O95821; Q5T8J4; Q9Y429 

Genbank Protein ID

 AF097725; AK023092; AL160163; BC012618; BC019866; BC032552; BC040950; AL050196 

Genbank Nucleotide ID

 AAD16169.1; BAB14399.1; CAI23487.1; AAH12618.1; AAH19866.1; AAH32552.1; AAH40950.1; CAB43314.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 20 

Protein Synonyms/Alias

 Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP 

Gene Name

 MED20 

Gene Synonyms/Alias

 TRFP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	QMPVAEGKSVQQTVE	ubiquitination	[1]
27	TVELLTRKLEMLGAE	ubiquitination	[1]
99	DVLMVKLKGFFQSAK	ubiquitination	[1]
202	ELFNKIRKQQQVPVA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

  

Keyword

 Activator; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 212 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; NAS:UniProtKB.
 GO:0003899; F:DNA-directed RNA polymerase activity; NAS:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR013921; Mediator_Med20. 

Pfam

 PF08612; Med20 

SMART

  

PROSITE

  

PRINTS