CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005701
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium/calmodulin-dependent protein kinase type II subunit beta 
Protein Synonyms/Alias
 CaM kinase II subunit beta; CaMK-II subunit beta 
Gene Name
 Camk2b 
Gene Synonyms/Alias
 Camk2d 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
22QLYEDIGKGAFSVVRubiquitination[1]
33SVVRRCVKLCTGHEYubiquitination[1]
43TGHEYAAKIINTKKLubiquitination[1]
69ARICRLLKHSNIVRLubiquitination[1]
147ENLLLASKCKGAAVKubiquitination[1]
259MLTINPAKRITAHEAubiquitination[1]
268ITAHEALKHPWVCQRubiquitination[1]
469YFENLLAKNSKPIHTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. 
Sequence Annotation
 DOMAIN 14 272 Protein kinase.
 NP_BIND 20 28 ATP (By similarity).
 REGION 283 292 Autoinhibitory domain (By similarity).
 REGION 291 301 Calmodulin-binding.
 ACT_SITE 136 136 Proton acceptor (By similarity).
 BINDING 43 43 ATP (By similarity).
 MOD_RES 17 17 Phosphotyrosine.
 MOD_RES 287 287 Phosphothreonine; by autocatalysis.
 MOD_RES 306 306 Phosphothreonine; by autocatalysis (By
 MOD_RES 307 307 Phosphothreonine; by autocatalysis (By
 MOD_RES 315 315 Phosphoserine.
 MOD_RES 367 367 Phosphoserine.
 MOD_RES 397 397 Phosphoserine.  
Keyword
 ATP-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Differentiation; Direct protein sequencing; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Reference proteome; Sarcoplasmic reticulum; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 542 AA 
Protein Sequence
MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKL SARDHQKLER 60
EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 120
LEAVLHCHQM GVVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 180
LSPEVLRKEA YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT 240
VTPEAKNLIN QMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKL 300
KGAILTTMLA TRNFSVGRQT TAPATMSTAA SGTTMGLVEQ AKSLLNKKAD GVKPQTNSTK 360
NSSAITSPKG SLPPAALEPQ TTVIHNPVDG IKESSDSTNT TIEDEDAKAR KQEIIKTTEQ 420
LIEAVNNGDF EAYAKICDPG LTSFEPEALG NLVEGMDFHR FYFENLLAKN SKPIHTTILN 480
PHVHVIGEDA ACIAYIRLTQ YIDGQGRPRT SQSEETRVWH RRDGKWQNVH FHCSGAPVAP 540
LQ 542 
Gene Ontology
 GO:0005954; C:calcium- and calmodulin-dependent protein kinase complex; TAS:UniProtKB.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0051233; C:spindle midzone; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IDA:MGI.
 GO:0060466; P:activation of meiosis involved in egg activation; IMP:MGI.
 GO:0006816; P:calcium ion transport; IMP:MGI.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:MGI.
 GO:0002030; P:inhibitory G-protein coupled receptor phosphorylation; IDA:MGI.
 GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:MGI.
 GO:2001235; P:positive regulation of apoptotic signaling pathway; IDA:MGI.
 GO:0061003; P:positive regulation of dendritic spine morphogenesis; ISS:UniProtKB.
 GO:0010976; P:positive regulation of neuron projection development; ISS:UniProtKB.
 GO:0090129; P:positive regulation of synapse maturation; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IMP:MGI.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IMP:MGI.
 GO:0032222; P:regulation of synaptic transmission, cholinergic; IMP:MGI.
 GO:0046686; P:response to cadmium ion; IDA:MGI. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR013543; Ca/CaM-dep_prot_kinase-assoc.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF08332; CaMKII_AD
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS