UniProt Accession

 MLX_HUMAN; Q9UH92; A8K2J3; B2RAV8; B2RD73; Q53XM6; Q96FL2; Q9H2V0; Q9H2V1; Q9H2V2; Q9NXN3 

Genbank Protein ID

 AF203978; AF213666; AF213667; AF213668; AK000150; AK290258; AK314378; AK315432; BT009812; BC010689 

Genbank Nucleotide ID

 AAF14638.1; AAG40145.1; AAG40146.1; AAG40147.1; BAA90977.1; BAF82947.1; BAG37005.1; BAG37820.1; AAP88814.1; AAH10689.1 

Protein Name

 Max-like protein X 

Protein Synonyms/Alias

 Class D basic helix-loop-helix protein 13; bHLHd13; Max-like bHLHZip protein; Protein BigMax; Transcription factor-like protein 4 

Gene Name

 MLX 

Gene Synonyms/Alias

 BHLHD13; TCFL4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
170	DFSIGSQKLSKAIVL	ubiquitination	[1]
173	IGSQKLSKAIVLQKT	ubiquitination	[1]
179	SKAIVLQKTIDYIQF	ubiquitination	[1]
192	QFLHKEKKKQEEEVS	ubiquitination	[1]
193	FLHKEKKKQEEEVST	ubiquitination	[1]
212	VTALKIMKVNYEQIV	ubiquitination	[1]
220	VNYEQIVKAHQDNPH	ubiquitination	[2]
294	IGVLHQLKNQLY***	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. 

Sequence Annotation

 DOMAIN 129 187 bHLH.
 REGION 140 160 Leucine-zipper.
 MOD_RES 45 45 Phosphoserine (By similarity).
 MOD_RES 48 48 Phosphoserine (By similarity).  

Keyword

 Activator; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 298 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0006913; P:nucleocytoplasmic transport; IEA:Compara.
 GO:0031325; P:positive regulation of cellular metabolic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS