UniProt Accession

 NARFL_HUMAN; Q9H6Q4; A1L385; B3KTJ3; Q53GC6; Q96S10; Q9H6J8 

Genbank Protein ID

 AY129231; AK025641; AK025861; AK095675; AK223005; AE006464; Z98258; CH471112; BC030248; BC129940 

Genbank Nucleotide ID

 AAM98737.1; BAB15199.1; BAB15261.1; BAG53105.1; BAD96725.1; AAK61251.1; CAM26480.1; EAW85723.1; AAH30248.1; AAI29941.1 

Protein Name

 Cytosolic Fe-S cluster assembly factor NARFL 

Protein Synonyms/Alias

 Iron-only hydrogenase-like protein 1; IOP1; Nuclear prelamin A recognition factor-like protein; Protein related to Narf 

Gene Name

 NARFL 

Gene Synonyms/Alias

 PRN 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
102	KKVLDANKMAAPSQQ	ubiquitination	[1, 2]
138	NPTDTARKLTSFFKK	ubiquitination	[2]
224	QVMGSLVKDFFAQQQ	ubiquitination	[1, 2]
237	QQHLTPDKIYHVTVM	ubiquitination	[2]
250	VMPCYDKKLEASRPD	ubiquitination	[2]
342	HVAEVTYKPLRNKDF	ubiquitination	[2, 3]
347	TYKPLRNKDFQEVTL	ubiquitination	[1, 2]
467	TQYHAVEKASTGLGI	ubiquitination	[2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. 

Sequence Annotation

 METAL 24 24 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 71 71 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 74 74 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 77 77 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 190 190 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 246 246 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 395 395 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 399 399 Iron-sulfur 2 (4Fe-4S) (Potential).
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 4Fe-4S; Acetylation; Alternative splicing; Complete proteome; Iron; Iron-sulfur; Metal-binding; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 476 AA 

Protein Sequence

Gene Ontology

 GO:0097361; C:CIA complex; IDA:UniProtKB.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016226; P:iron-sulfur cluster assembly; IMP:UniProtKB.
 GO:0032364; P:oxygen homeostasis; IDA:HGNC.
 GO:0006355; P:regulation of transcription, DNA-dependent; IDA:HGNC.
 GO:0001666; P:response to hypoxia; IDA:HGNC.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR009016; Fe_hydrogenase.
 IPR004108; Fe_hydrogenase_lsu_C.
 IPR003149; Fe_hydrogenase_ssu-like. 

Pfam

 PF02906; Fe_hyd_lg_C
 PF02256; Fe_hyd_SSU 

SMART

 SM00902; Fe_hyd_SSU 

PROSITE

  

PRINTS