CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008520
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 D-lactate dehydrogenase 
Protein Synonyms/Alias
 D-LDH; Fermentative lactate dehydrogenase 
Gene Name
 ldhA 
Gene Synonyms/Alias
 hslI; htpH; b1380; JW1375 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
9KLAVYSTKQYDKKYLacetylation[1]
13YSTKQYDKKYLQQVNacetylation[1]
70ELKKHGVKYIALRCAacetylation[1]
88NVDLDAAKELGLKVVacetylation[1]
93AAKELGLKVVRVPAYacetylation[1]
154AGVIGTGKIGVAMLRacetylation[1]
164VAMLRILKGFGMRLLacetylation[1]
248QAAIEALKNQKIGSLacetylation[1]
271RDLFFEDKSNDVIQDacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Fermentative lactate dehydrogenase. 
Sequence Annotation
 NP_BIND 154 155 NAD (By similarity).
 NP_BIND 232 234 NAD (By similarity).
 NP_BIND 295 298 NAD (By similarity).
 ACT_SITE 234 234 By similarity.
 ACT_SITE 263 263 By similarity.
 ACT_SITE 295 295 Proton donor (By similarity).
 BINDING 258 258 NAD (By similarity).  
Keyword
 Complete proteome; NAD; Oxidoreductase; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 329 AA 
Protein Sequence
MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI FVNDDGSRPV 60
LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY DPEAVAEHAI GMMMTLNRRI 120
HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI GTGKIGVAML RILKGFGMRL LAFDPYPSAA 180
ALELGVEYVD LPTLFSESDV ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS 240
QAAIEALKNQ KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT 300
AEALTSISQT TLQNLSNLEK GETCPNELV 329 
Gene Ontology
 GO:0008720; F:D-lactate dehydrogenase activity; IDA:EcoCyc.
 GO:0070404; F:NADH binding; IDA:EcoliWiki.
 GO:0019664; P:glucose catabolic process to mixed acids; IDA:EcoCyc.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 
Interpro
 IPR006139; D-isomer_2_OHA_DH_cat_dom.
 IPR006140; D-isomer_2_OHA_DH_NAD-bd.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00389; 2-Hacid_dh
 PF02826; 2-Hacid_dh_C 
SMART
  
PROSITE
 PS00065; D_2_HYDROXYACID_DH_1
 PS00670; D_2_HYDROXYACID_DH_2
 PS00671; D_2_HYDROXYACID_DH_3 
PRINTS