UniProt Accession

 NASP_RAT; Q66HD3 

Genbank Protein ID

 BC081913 

Genbank Nucleotide ID

 AAH81913.1 

Protein Name

 Nuclear autoantigenic sperm protein 

Protein Synonyms/Alias

 NASP 

Gene Name

 Nasp 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
154	VYDAMGEKEAKKAEG	acetylation	[1]
197	PAEKLQEKVESTSKQ	acetylation	[1]
203	EKVESTSKQLTESSE	acetylation	[1]
229	EDEVTSGKTEQESLC	acetylation	[1]
241	SLCTEEGKSISGVYV	acetylation	[1]
251	SGVYVQNKEFREAVP	acetylation	[1]
286	QTIGAPEKQDTLMKV	acetylation	[1]
443	TEKAPEDKFKIAANE	acetylation	[1]
523	IFKRQETKEAQLYAA	acetylation	[1]
604	KSIDVIEKRMAVLLE	acetylation	[1]
631	EKEIEELKELLPEIR	acetylation	[1]
645	REKIEDAKESQRSGN	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity). 

Sequence Annotation

 REPEAT 43 76 TPR 1.
 REPEAT 530 563 TPR 2.
 REPEAT 572 605 TPR 3.
 REGION 116 127 Histone-binding (By similarity).
 REGION 210 242 Histone-binding (By similarity).
 REGION 457 500 Histone-binding (By similarity).
 MOTIF 704 710 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 33 33 N6-acetyllysine (By similarity).
 MOD_RES 123 123 Phosphothreonine (By similarity).
 MOD_RES 127 127 Phosphoserine (By similarity).
 MOD_RES 188 188 Phosphoserine (By similarity).
 MOD_RES 242 242 Phosphoserine (By similarity).
 MOD_RES 321 321 Phosphoserine (By similarity).
 MOD_RES 379 379 Phosphothreonine (By similarity).
 MOD_RES 452 452 Phosphothreonine (By similarity).
 MOD_RES 465 465 Phosphothreonine (By similarity).
 MOD_RES 468 468 Phosphoserine (By similarity).
 MOD_RES 485 485 Phosphoserine (By similarity).
 MOD_RES 671 671 Phosphothreonine (By similarity).
 MOD_RES 714 714 Phosphoserine (By similarity).
 MOD_RES 739 739 Phosphoserine (By similarity).  

Keyword

 Acetylation; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; DNA replication; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; TPR repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 776 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0051879; F:Hsp90 protein binding; ISS:UniProtKB.
 GO:0001824; P:blastocyst development; ISS:UniProtKB.
 GO:0007049; P:cell cycle; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; ISS:UniProtKB.
 GO:0006260; P:DNA replication; ISS:UniProtKB.
 GO:0043486; P:histone exchange; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR019544; Tetratricopeptide_SHNi-TPR_dom.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF10516; SHNi-TPR
 PF00515; TPR_1 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS