CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012532
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Interferon regulatory factor 4 
Protein Synonyms/Alias
 IRF-4; Lymphocyte-specific interferon regulatory factor; LSIRF; Multiple myeloma oncogene 1; NF-EM5 
Gene Name
 IRF4 
Gene Synonyms/Alias
 MUM1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103RLRCALNKSNDFEELubiquitination[1]
123LDISDPYKVYRIVPEubiquitination[1]
264YYREILVKELTTSSPubiquitination[1]
327APDGLYAKRLCQSRIubiquitination[1]
357LERDQTCKLFDTQQFubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). 
Sequence Annotation
 DNA_BIND 21 129 IRF tryptophan pentad repeat.
 MOD_RES 447 447 Phosphoserine; by ROCK2 (By similarity).
 MOD_RES 448 448 Phosphoserine; by ROCK2 (By similarity).  
Keyword
 3D-structure; Activator; Alternative splicing; Chromosomal rearrangement; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 451 AA 
Protein Sequence
MNLEGGGRGG EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSIF RIPWKHAGKQ 60
DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA LNKSNDFEEL VERSQLDISD 120
PYKVYRIVPE GAKKGAKQLT LEDPQMSMSH PYTMTTPYPS LPAQQVHNYM MPPLDRSWRD 180
YVPDQPHPEI PYQCPMTFGP RGHHWQGPAC ENGCQVTGTF YACAPPESQA PGVPTEPSIR 240
SAEALAFSDC RLHICLYYRE ILVKELTTSS PEGCRISHGH TYDASNLDQV LFPYPEDNGQ 300
RKNIEKLLSH LERGVVLWMA PDGLYAKRLC QSRIYWDGPL ALCNDRPNKL ERDQTCKLFD 360
TQQFLSELQA FAHHGRSLPR FQVTLCFGEE FPDPQRQRKL ITAHVEPLLA RQLYYFAQQN 420
SGHFLRGYDL PEHISNPEDY HRSIRHSSIQ E 451 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000788; C:nuclear nucleosome; IEA:Compara.
 GO:0005634; C:nucleus; IC:UniProtKB.
 GO:0000975; F:regulatory region DNA binding; IEA:InterPro.
 GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0008134; F:transcription factor binding; NAS:UniProtKB.
 GO:0042832; P:defense response to protozoan; ISS:UniProtKB.
 GO:0043966; P:histone H3 acetylation; IEA:Compara.
 GO:0043967; P:histone H4 acetylation; IEA:Compara.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0043011; P:myeloid dendritic cell differentiation; IEA:Compara.
 GO:0034122; P:negative regulation of toll-like receptor signaling pathway; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
 GO:0045082; P:positive regulation of interleukin-10 biosynthetic process; IDA:UniProtKB.
 GO:0045368; P:positive regulation of interleukin-13 biosynthetic process; IDA:UniProtKB.
 GO:0045086; P:positive regulation of interleukin-2 biosynthetic process; IDA:UniProtKB.
 GO:0045404; P:positive regulation of interleukin-4 biosynthetic process; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0045622; P:regulation of T-helper cell differentiation; NAS:UniProtKB.
 GO:0072540; P:T-helper 17 cell lineage commitment; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 
Interpro
 IPR019817; Interferon_reg_fac_CS.
 IPR001346; Interferon_reg_fact_DNA-bd_dom.
 IPR019471; Interferon_reg_factor-3.
 IPR017855; SMAD_dom-like.
 IPR008984; SMAD_FHA_domain.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00605; IRF
 PF10401; IRF-3 
SMART
 SM00348; IRF 
PROSITE
 PS00601; IRF_1
 PS51507; IRF_2 
PRINTS
 PR00267; INTFRNREGFCT.