| Tag | Content |
|---|
| CPLM ID | CPLM-001665 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3 |
Protein Synonyms/Alias | Glucosaminyl N-deacetylase/N-sulfotransferase 3; NDST-3; hNDST-3; N-heparan sulfate sulfotransferase 3; N-HSST 3; Heparan sulfate N-deacetylase 3; Heparan sulfate N-sulfotransferase 3 |
Gene Name | NDST3 |
Gene Synonyms/Alias | HSST3; UNQ2544/PRO4998 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 327 | RMNTNDVKALLDTQN | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity. |
Sequence Annotation | NP_BIND 605 609 PAPS (By similarity).
NP_BIND 824 828 PAPS (By similarity).
REGION 36 589 Heparan sulfate N-deacetylase 3.
REGION 590 873 Heparan sulfate N-sulfotransferase 3.
ACT_SITE 605 605 For sulfotransferase activity (By
BINDING 703 703 PAPS (By similarity).
CARBOHYD 146 146 N-linked (GlcNAc...) (Potential).
CARBOHYD 226 226 N-linked (GlcNAc...) (Potential).
CARBOHYD 342 342 N-linked (GlcNAc...) (Potential).
CARBOHYD 392 392 N-linked (GlcNAc...) (Potential).
CARBOHYD 658 658 N-linked (GlcNAc...) (Potential).
CARBOHYD 794 794 N-linked (GlcNAc...) (Potential).
DISULFID 809 819 By similarity. |
Keyword | Alternative splicing; Complete proteome; Disulfide bond; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Multifunctional enzyme; Polymorphism; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 873 AA |
Protein Sequence | MSFIMKLHRH FQRTVILLAT FCMVSIIISA YYLYSGYKQE NELSETASEV DCGDLQHLPY 60
QLMEVKAMKL FDASRTDPTV LVFVESQYSS LGQDIIMILE SSRFQYHIEI APGKGDLPVL 120
IDKMKGKYIL IIYENILKYI NMDSWNRSLL DKYCVEYGVG VIGFHKTSEK SVQSFQLKGF 180
PFSIYGNLAV KDCCINPHSP LIRVTKSSKL EKGSLPGTDW TVFQINHSAY QPVIFAKVKT 240
PENLSPSISK GAFYATIIHD LGLHDGIQRV LFGNNLNFWL HKLIFIDAIS FLSGKRLTLS 300
LDRYILVDID DIFVGKEGTR MNTNDVKALL DTQNLLRAQI TNFTFNLGFS GKFYHTGTEE 360
EDEGDDCLLG SVDEFWWFPH MWSHMQPHLF HNESSLVEQM ILNKKFALEH GIPTDMGYAV 420
APHHSGVYPV HVQLYEAWKK VWNIKITSTE EYPHLKPARY RRGFIHKNIM VLPRQTCGLF 480
THTIFYKEYP GGPKELDKSI QGGELFFTVV LNPISIFMTH LSNYGNDRLG LYTFVNLANF 540
VKSWTNLRLQ TLPPVQLAHK YFELFPDQKD PLWQNPCDDK RHRDIWSKEK TCDRLPKFLV 600
IGPQKTGTTA LYLFLVMHPS ILSNSPSPKT FEEVQFFNRN NYHRGIDWYM DFFPVPSNVT 660
TDFLFEKSAN YFHSEEAPKR AASLVPKAKI ITILIDPSDR AYSWYQHQRS HEDPAALKFS 720
FYEVISAGPR APSELRALQK RCLVPGWYAS HIERWLVYFP PFQLLIIDGQ QLRTDPATVM 780
DEVQKFLGVL PHYNYSEALT FDSHKGFWCQ LLEEGKTKCL GKSKGRKYPP MDSDSRTFLS 840
SYYRDHNVEL SKLLHKLGQP LPSWLRQELQ KVR 873 |
Gene Ontology | GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell. GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IEA:EC. GO:0019213; F:deacetylase activity; IEA:Compara. GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IEA:UniProtKB-UniPathway. GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |