CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007511
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 
Protein Synonyms/Alias
 General control non-derepressible protein 10; Protein GCD10; tRNA(m1A58)-methyltransferase subunit TRM6; tRNA(m1A58)MTase subunit TRM6 
Gene Name
 GCD10 
Gene Synonyms/Alias
 TIF33; TRM6; YNL062C; N2422 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
160SSKEIIDKIIKSHKSacetylation[1]
171SHKSFHNKTVYSQEKacetylation[1]
436LHPLMTMKGGGGYLMmethylation[2]
467TAADSSEKLAEHGAKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 
Functional Description
 Substrate-binding subunit of tRNA (adenine-N(1)-)- methyltransferase, which catalyzes the formation of N(1)- methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency. 
Sequence Annotation
  
Keyword
 Complete proteome; Nucleus; Reference proteome; Repressor; RNA-binding; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 478 AA 
Protein Sequence
MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP FGLTFEIYYD 60
GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG QSEPPLSIKE KSVSLELSSI 120
DSSATNQNLV NMGSKAQELT VEEIEKMKQE SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV 180
NRKKQKFAKY FTVEYLSSSN LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM 240
DETGGLLVYF LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS 300
LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL ELTGEFLYDG 360
LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA HTLYSDLRFL APSILETRCR 420
PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP APEPVSENAT AADSSEKLAE HGAKKQKI 478 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0031515; C:tRNA (m1A) methyltransferase complex; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IEA:InterPro.
 GO:0006446; P:regulation of translational initiation; IEA:InterPro.
 GO:0030488; P:tRNA methylation; IDA:SGD. 
Interpro
 IPR007316; EIF3_gamma.
 IPR017423; tRNA_m1A_mtfrase. 
Pfam
 PF04189; Gcd10p 
SMART
  
PROSITE
  
PRINTS