UniProt Accession

 SERC_ECOLI; P23721; P78266 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC73993.1; BAA35642.1 

Protein Name

 Phosphoserine aminotransferase 

Protein Synonyms/Alias

 Phosphohydroxythreonine aminotransferase; PSAT 

Gene Name

 serC 

Gene Synonyms/Alias

 pdxC; pdxF; b0907; JW0890 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
20	MLPAEVLKQAQQELR	acetylation	[1]
44	MEVSHRGKEFIQVAE	acetylation	[2]
55	QVAEEAEKDFRDLLN	acetylation	[2]
68	LNVPSNYKVLFCHGG	acetylation	[2]
91	PLNILGDKTTADYVD	acetylation	[2]
108	YWAASAIKEAKKYCT	acetylation	[1, 2]
111	ASAIKEAKKYCTPNV	acetylation	[2]
122	TPNVFDAKVTVDGLR	acetylation	[2]
257	GLVFKWLKANGGVAE	acetylation	[2]
267	GGVAEMDKINQQKAE	acetylation	[2]
292	FYRNDVAKANRSRMN	acetylation	[2]
311	LADSALDKLFLEESF	acetylation	[2]
326	AAGLHALKGHRVVGG	acetylation	[1, 2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. Is involved in both pyridoxine and serine biosynthesis. 

Sequence Annotation

 REGION 76 77 Pyridoxal phosphate binding.
 REGION 239 240 Pyridoxal phosphate binding.
 BINDING 9 9 L-glutamate.
 BINDING 42 42 L-glutamate.
 BINDING 102 102 Pyridoxal phosphate.
 BINDING 153 153 Pyridoxal phosphate.
 BINDING 174 174 Pyridoxal phosphate.
 BINDING 197 197 Pyridoxal phosphate.
 MOD_RES 198 198 N6-(pyridoxal phosphate)lysine.  

Keyword

 3D-structure; Amino-acid biosynthesis; Aminotransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Pyridoxal phosphate; Pyridoxine biosynthesis; Reference proteome; Serine biosynthesis; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 362 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004648; F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity; IDA:EcoCyc.
 GO:0030170; F:pyridoxal phosphate binding; IEA:HAMAP.
 GO:0006564; P:L-serine biosynthetic process; IMP:EcoCyc.
 GO:0042823; P:pyridoxal phosphate biosynthetic process; IMP:EcoCyc.
 GO:0008615; P:pyridoxine biosynthetic process; IEA:HAMAP. 

Interpro

 IPR000192; Aminotrans_V/Cys_dSase.
 IPR020578; Aminotrans_V_PyrdxlP_BS.
 IPR022278; Pser_aminoTfrase.
 IPR003248; Pser_aminoTfrase_subgr.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF00266; Aminotran_5 

SMART

  

PROSITE

 PS00595; AA_TRANSFER_CLASS_5 

PRINTS