UniProt Accession

 SELB_MOUSE; Q9JHW4; Q6GTZ5 

Genbank Protein ID

 AF283518; AF268871; AK028424; CH466523; BC024915 

Genbank Nucleotide ID

 AAF91471.1; AAG13374.1; BAC25942.1; EDK99245.1; AAH24915.1 

Protein Name

 Selenocysteine-specific elongation factor 

Protein Synonyms/Alias

 Elongation factor sec; Eukaryotic elongation factor, selenocysteine-tRNA-specific; mSelB 

Gene Name

 Eefsec 

Gene Synonyms/Alias

 Selb 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
38	ASTAAFDKQPQSRER	ubiquitination	[1]
141	IDLLAEGKRQAAIDK	ubiquitination	[1]
254	LKVVKKVKSMQMFHT	ubiquitination	[1]
307	AALISVEKIPYFRGP	acetylation	[2, 3]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP. 

Sequence Annotation

 NP_BIND 14 21 GTP (Potential).
 NP_BIND 78 82 GTP (Potential).
 NP_BIND 132 136 GTP (Potential).
 MOTIF 534 540 Nuclear localization signal (Potential).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Nucleus; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 583 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IDA:MGI.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IDA:MGI.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0001514; P:selenocysteine incorporation; IDA:MGI. 

Interpro

 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 

Pfam

 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2 

SMART

  

PROSITE

 PS00301; EFACTOR_GTP 

PRINTS

 PR00315; ELONGATNFCT.