UniProt Accession

 GALT2_HUMAN; Q10471; A8K1Y3; C5HU00; Q9NPY4 

Genbank Protein ID

 X85019; AK290048; AL078646; AL117349; AL136988; AL117349; AL078646; AL136988; AL136988; AL078646; AL117349; FJ515852; CH471098; BC041120 

Genbank Nucleotide ID

 CAA59381.1; BAF82737.1; CAC00585.2; CAC00585.2; CAC00585.2; CAI22902.1; CAI22902.1; CAI22902.1; CAI23447.1; CAI23447.1; CAI23447.1; ACS13744.1; EAW69911.1; AAH41120.1 

Protein Name

 Polypeptide N-acetylgalactosaminyltransferase 2 

Protein Synonyms/Alias

 Polypeptide GalNAc transferase 2; GalNAc-T2; pp-GaNTase 2; Protein-UDP acetylgalactosaminyltransferase 2; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2; Polypeptide N-acetylgalactosaminyltransferase 2 soluble form 

Gene Name

 GALNT2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
53	NEIDPIKKKDLHHSN	ubiquitination	[1]
103	QDPYARNKFNQVESD	acetylation	[2]
103	QDPYARNKFNQVESD	ubiquitination	[1]
111	FNQVESDKLRMDRAI	acetylation	[2]
111	FNQVESDKLRMDRAI	ubiquitination	[1]
162	TVVSVLKKSPPHLIK	ubiquitination	[1]
189	EDGALLGKIEKVRVL	ubiquitination	[1, 3]
218	GADAAQAKVLTFLDS	ubiquitination	[1]
302	GNPVAPIKTPMIAGG	ubiquitination	[1]
363	RVGHVFRKQHPYTFP	acetylation	[2]
363	RVGHVFRKQHPYTFP	ubiquitination	[1]
393	EVWMDEYKNFYYAAV	ubiquitination	[1]
509	RAPGSLIKLQGCREN	ubiquitination	[1]
521	RENDSRQKWEQIEGN	acetylation	[2]
521	RENDSRQKWEQIEGN	ubiquitination	[1]
530	EQIEGNSKLRHVGSN	ubiquitination	[1]
546	CLDSRTAKSGGLSVE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Catalytic and glycan-binding abilities of ppGalNAc-T2 are regulated by acetylation.
 Zlocowski N, Sendra VG, Lorenz V, Villarreal MA, Jorge A, Núñez Y, Bennett EP, Clausen H, Nores GA, Irazoqui FJ.
 Biochem Biophys Res Commun. 2011 Jun 24;410(1):140-5. [PMID: 21651894]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. 

Sequence Annotation

 DOMAIN 443 566 Ricin B-type lectin.
 REGION 135 240 Catalytic subdomain A.
 REGION 300 362 Catalytic subdomain B.
 DISULFID 456 473 By similarity.
 DISULFID 496 513 By similarity.
 DISULFID 539 555 By similarity.  

Keyword

 3D-structure; Calcium; Complete proteome; Direct protein sequencing; Disulfide bond; Glycosyltransferase; Golgi apparatus; Lectin; Manganese; Membrane; Polymorphism; Reference proteome; Secreted; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 571 AA 

Protein Sequence

Gene Ontology

 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0005795; C:Golgi stack; IDA:BHF-UCL.
 GO:0030173; C:integral to Golgi membrane; NAS:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0030145; F:manganese ion binding; IDA:BHF-UCL.
 GO:0004653; F:polypeptide N-acetylgalactosaminyltransferase activity; IDA:BHF-UCL.
 GO:0002378; P:immunoglobulin biosynthetic process; IDA:BHF-UCL.
 GO:0016266; P:O-glycan processing; TAS:Reactome.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018242; P:protein O-linked glycosylation via serine; IDA:BHF-UCL.
 GO:0018243; P:protein O-linked glycosylation via threonine; IDA:BHF-UCL. 

Interpro

 IPR001173; Glyco_trans_2.
 IPR000772; Ricin_B_lectin. 

Pfam

 PF00535; Glycos_transf_2
 PF00652; Ricin_B_lectin 

SMART

 SM00458; RICIN 

PROSITE

 PS50231; RICIN_B_LECTIN 

PRINTS