CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004464
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADPH--cytochrome P450 reductase 
Protein Synonyms/Alias
 CPR; P450R 
Gene Name
 POR 
Gene Synonyms/Alias
 CYPOR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56EEVPEFTKIQTLTSSubiquitination[1]
487TKAGRINKGVATNWLubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. 
Sequence Annotation
 DOMAIN 80 224 Flavodoxin-like.
 DOMAIN 279 521 FAD-binding FR-type.
 NP_BIND 170 201 FMN (By similarity).
 NP_BIND 314 325 FAD (By similarity).
 NP_BIND 451 461 FAD (By similarity).
 NP_BIND 529 547 NADP (By similarity).
 NP_BIND 623 639 NADP (By similarity).
 MOD_RES 2 2 N-acetylglycine.
 MOD_RES 575 575 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Congenital adrenal hyperplasia; Craniosynostosis; Direct protein sequencing; Disease mutation; Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MGDSHVDTSS TVSEAVAEEV SLFSMTDMIL FSLIVGLLTY WFLFRKKKEE VPEFTKIQTL 60
TSSVRESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM SADPEEYDLA 120
DLSSLPEIDN ALVVFCMATY GEGDPTDNAQ DFYDWLQETD VDLSGVKFAV FGLGNKTYEH 180
FNAMGKYVDK RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES 240
SIRQYELVVH TDIDAAKVYM GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 300
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGKILGA DLDVVMSLNN LDEESNKKHP 360
FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE LLRKMASSSG EGKELYLSWV 420
VEARRHILAI LQDCPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVVVEYET 480
KAGRINKGVA TNWLRAKEPA GENGGRALVP MFVRKSQFRL PFKATTPVIM VGPGTGVAPF 540
IGFIQERAWL RQQGKEVGET LLYYGCRRSD EDYLYREELA QFHRDGALTQ LNVAFSREQS 600
HKVYVQHLLK QDREHLWKLI EGGAHIYVCG DARNMARDVQ NTFYDIVAEL GAMEHAQAVD 660
YIKKLMTKGR YSLDVWS 677 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:Compara.
 GO:0009055; F:electron carrier activity; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0010181; F:FMN binding; IEA:Compara.
 GO:0016787; F:hydrolase activity; IEA:Compara.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0047726; F:iron-cytochrome-c reductase activity; IEA:Compara.
 GO:0050661; F:NADP binding; IEA:Compara.
 GO:0003958; F:NADPH-hemoprotein reductase activity; TAS:UniProtKB.
 GO:0009437; P:carnitine metabolic process; IEA:Compara.
 GO:0090346; P:cellular organofluorine metabolic process; IDA:BHF-UCL.
 GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEA:Compara.
 GO:0071375; P:cellular response to peptide hormone stimulus; IEA:Compara.
 GO:0070988; P:demethylation; IEA:Compara.
 GO:0019395; P:fatty acid oxidation; IEA:Compara.
 GO:0009812; P:flavonoid metabolic process; IEA:Compara.
 GO:0018393; P:internal peptidyl-lysine acetylation; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0060192; P:negative regulation of lipase activity; IEA:Compara.
 GO:0043602; P:nitrate catabolic process; IEA:Compara.
 GO:0046210; P:nitric oxide catabolic process; IEA:Compara.
 GO:0045542; P:positive regulation of cholesterol biosynthetic process; IEA:Compara.
 GO:0032332; P:positive regulation of chondrocyte differentiation; IEA:Compara.
 GO:0032770; P:positive regulation of monooxygenase activity; IDA:BHF-UCL.
 GO:0045880; P:positive regulation of smoothened signaling pathway; IEA:Compara.
 GO:0090031; P:positive regulation of steroid hormone biosynthetic process; IEA:Compara.
 GO:0003420; P:regulation of growth plate cartilage chondrocyte proliferation; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara. 
Interpro
 IPR003097; FAD-binding_1.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001094; Flavdoxin.
 IPR008254; Flavodoxin/NO_synth.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR023208; P450R.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00667; FAD_binding_1
 PF00258; Flavodoxin_1
 PF00175; NAD_binding_1 
SMART
  
PROSITE
 PS51384; FAD_FR
 PS50902; FLAVODOXIN_LIKE 
PRINTS
 PR00369; FLAVODOXIN.
 PR00371; FPNCR.