CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001797
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase I 
Protein Synonyms/Alias
 POL I 
Gene Name
 polA 
Gene Synonyms/Alias
 resA; b3863; JW3835 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
54RSLIMQYKPTHAAVVacetylation[1]
65AAVVFDAKGKTFRDEacetylation[1]
67VVFDAKGKTFRDELFacetylation[1]
127TLAREAEKAGRPVLIacetylation[1]
139VLISTGDKDMAQLVTacetylation[1]
230GLSFRGAKTMAAKLEacetylation[1]
235GAKTMAAKLEQNKEVacetylation[1]
240AAKLEQNKEVAYLSYacetylation[1]
295TADVEAGKWLQAKGAacetylation[1]
345TLKAWIAKLEKAPVFacetylation[1]
406ERALELLKPLLEDEKacetylation[1]
413KPLLEDEKALKVGQNacetylation[1]
422LKVGQNLKYDRGILAacetylation[1]
481EEIAGKGKNQLTFNQacetylation[1]
518KMWPDLQKHKGPLNVacetylation[1]
520WPDLQKHKGPLNVFEacetylation[1]
546RIERNGVKIDPKVLHacetylation[1]
550NGVKIDPKVLHNHSEacetylation[1]
568LRLAELEKKAHEIAGacetylation[1]
593LQTILFEKQGIKPLKacetylation[1]
643LKSTYTDKLPLMINPacetylation[1]
721MAHLSRDKGLLTAFAacetylation[1]
731LTAFAEGKDIHRATAacetylation[1]
786IPRKEAQKYMDLYFEacetylation[1]
810ERTRAQAKEQGYVETacetylation[1]
829RLYLPDIKSSNGARRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template. 
Sequence Annotation
 DOMAIN 1 323 5'-3' exonuclease.
 DOMAIN 324 517 3'-5' exonuclease.
 REGION 324 928 Klenow fragment.
 REGION 521 928 Polymerase.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 928 AA 
Protein Sequence
MVQIPQNPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI MQYKPTHAAV 60
VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK AMGLPLLAVS GVEADDVIGT 120
LAREAEKAGR PVLISTGDKD MAQLVTPNIT LINTMTNTIL GPEEVVNKYG VPPELIIDFL 180
ALMGDSSDNI PGVPGVGEKT AQALLQGLGG LDTLYAEPEK IAGLSFRGAK TMAAKLEQNK 240
EVAYLSYQLA TIKTDVELEL TCEQLEVQQP AAEELLGLFK KYEFKRWTAD VEAGKWLQAK 300
GAKPAAKPQE TSVADEAPEV TATVISYDNY VTILDEETLK AWIAKLEKAP VFAFDTETDS 360
LDNISANLVG LSFAIEPGVA AYIPVAHDYL DAPDQISRER ALELLKPLLE DEKALKVGQN 420
LKYDRGILAN YGIELRGIAF DTMLESYILN SVAGRHDMDS LAERWLKHKT ITFEEIAGKG 480
KNQLTFNQIA LEEAGRYAAE DADVTLQLHL KMWPDLQKHK GPLNVFENIE MPLVPVLSRI 540
ERNGVKIDPK VLHNHSEELT LRLAELEKKA HEIAGEEFNL SSTKQLQTIL FEKQGIKPLK 600
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP KTGRVHTSYH 660
QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV IVSADYSQIE LRIMAHLSRD 720
KGLLTAFAEG KDIHRATAAE VFGLPLETVT SEQRRSAKAI NFGLIYGMSA FGLARQLNIP 780
RKEAQKYMDL YFERYPGVLE YMERTRAQAK EQGYVETLDG RRLYLPDIKS SNGARRAAAE 840
RAAINAPMQG TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDVDAVAKQI 900
HQLMENCTRL DVPLLVEVGS GENWDQAH 928 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0008408; F:3'-5' exonuclease activity; IDA:EcoCyc.
 GO:0008409; F:5'-3' exonuclease activity; IDA:EcoCyc.
 GO:0003677; F:DNA binding; IDA:EcoCyc.
 GO:0003887; F:DNA-directed DNA polymerase activity; IDA:EcoCyc.
 GO:0006281; P:DNA repair; IMP:EcoCyc.
 GO:0006261; P:DNA-dependent DNA replication; IDA:EcoCyc. 
Interpro
 IPR002562; 3'-5'_exonuclease_dom.
 IPR020046; 5-3_exonucl_a-hlix_arch_N.
 IPR020045; 5-3_exonuclease_C.
 IPR002421; 5-3_exonuclease_N.
 IPR019760; DNA-dir_DNA_pol_A_CS.
 IPR001098; DNA-dir_DNA_pol_A_palm_dom.
 IPR018320; DNA_polymerase_1.
 IPR002298; DNA_polymerase_A.
 IPR008918; HhH2.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF01367; 5_3_exonuc
 PF02739; 5_3_exonuc_N
 PF00476; DNA_pol_A
 PF01612; DNA_pol_A_exo1 
SMART
 SM00474; 35EXOc
 SM00475; 53EXOc
 SM00278; HhH1
 SM00279; HhH2
 SM00482; POLAc 
PROSITE
 PS00447; DNA_POLYMERASE_A 
PRINTS
 PR00868; DNAPOLI.