CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012738
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate carboxykinase [GTP], mitochondrial 
Protein Synonyms/Alias
 PEPCK-M; Phosphoenolpyruvate carboxylase 
Gene Name
 PCK2 
Gene Synonyms/Alias
 PEPCK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108DVARVESKTVIVTPSubiquitination[1]
294GITSPAGKKRYVAAAubiquitination[1]
578TPIGLVPKEGALDLSubiquitination[1]
600TQLFSLPKDFWEQEVubiquitination[1]
624QVNQDLPKEVLAELEacetylation[2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity). 
Sequence Annotation
 NP_BIND 305 310 GTP (By similarity).
 NP_BIND 548 551 GTP (By similarity).
 REGION 421 423 Substrate binding (By similarity).
 ACT_SITE 306 306 By similarity.
 METAL 262 262 Manganese (By similarity).
 METAL 282 282 Manganese (By similarity).
 METAL 329 329 Manganese (By similarity).
 BINDING 104 104 Substrate (By similarity).
 BINDING 255 255 Substrate; via amide nitrogen (By
 BINDING 262 262 Substrate (By similarity).
 BINDING 304 304 Substrate (By similarity).
 BINDING 423 423 GTP (By similarity).
 BINDING 454 454 GTP (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 640 AA 
Protein Sequence
MAALYRPGLR LNWHGLSPLG WPSCRSIQTL RVLSGDLGQL PTGIRDFVEH SARLCQPEGI 60
HICDGTEAEN TATLTLLEQQ GLIRKLPKYN NCWLARTDPK DVARVESKTV IVTPSQRDTV 120
QLPPGGARGQ LGNWMSPADF QRAVDERFPG CMQGRTMYVL PFSMGPVGSP LSRIGVQLTD 180
SAYVVASMRI MTRLGTPVLQ ALGDGDFVKC LHSVGQPLTG QGEPVSQWPC NPEKTLIGHV 240
PDQREIISFG SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITS PAGKKRYVAA 300
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF FGVAPGTSAT 360
TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT VTSWLGKPWK PGDKEPCAHP 420
NSRFCAPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS 480
ESTAAAEHKG KIIMHDPFAM RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA 540
GHFLWPGFGE NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK 600
DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM 640 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:EC.
 GO:0004611; F:phosphoenolpyruvate carboxykinase activity; TAS:ProtInc.
 GO:0006094; P:gluconeogenesis; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR018091; PEP_carboxykin_GTP_CS.
 IPR013035; PEP_carboxykinase_C.
 IPR008209; PEP_carboxykinase_GTP.
 IPR008210; PEP_carboxykinase_N. 
Pfam
 PF00821; PEPCK 
SMART
  
PROSITE
 PS00505; PEPCK_GTP 
PRINTS