CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011847
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diphthine--ammonia ligase 
Protein Synonyms/Alias
 Diphthamide synthase; Diphthamide synthetase 
Gene Name
 DPH6 
Gene Synonyms/Alias
 YLR143W; L3177 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
172NDTNSGNKFDARIIKubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (EFT1 or EFT2) to diphthamide. 
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 685 AA 
Protein Sequence
MKFIALISGG KDSFYNIFHC LKNNHELIAL GNIYPKESEE QELDSFMFQT VGHDLIDYYS 60
KCIGVPLFRR SILRNTSNNV ELNYTATQDD EIEELFELLR TVKDKIPDLE AVSVGAILSS 120
YQRTRVENVC SRLGLVVLSY LWQRDQAELM GEMCLMSKDV NNVENDTNSG NKFDARIIKV 180
AAIGLNEKHL GMSLPMMQPV LQKLNQLYQV HICGEGGEFE TMVLDAPFFQ HGYLELIDIV 240
KCSDGEVHNA RLKVKFQPRN LSKSFLLNQL DQLPVPSIFG NNWQDLTQNL PKQQAKTGEQ 300
RFENHMSNAL PQTTINKTND KLYISNLQSR KSETVEKQSE DIFTELADIL HSNQIPRNHI 360
LSASLLIRDM SNFGKINKIY NEFLDLSKYG PLPPSRACVG SKCLPEDCHV QLSVVVDVKN 420
TGKEKINKNK GGLHVQGRSY WAPCNIGPYS QSTWLNDDAN QVSFISGQIG LVPQSMEILG 480
TPLTDQIVLA LQHFDTLCET IGAQEKLLMT CYISDESVLD SVIKTWAFYC SNMNHRSDLW 540
MDKSDDVEKC LVLVKISELP RGAVAEFGGV TCKRLIVDDN DSDKKEREEN DDVSTVFQKL 600
NLNIEGFHNT TVSAFGYNRN FITGFVDSRE ELELILEKTP KSAQITLYYN PKEIITFHHH 660
IGYYPVEKLF DYRGKEHRFG LHIRS 685 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0017178; F:diphthine-ammonia ligase activity; IMP:SGD.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IMP:SGD. 
Interpro
 IPR002761; DUF71_dom.
 IPR013813; Endoribo_LPSP/chorism_mut-like.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR006175; YjgF/Yer057p/UK114. 
Pfam
 PF01902; ATP_bind_4
 PF01042; Ribonuc_L-PSP 
SMART
  
PROSITE
 PS01094; UPF0076 
PRINTS