UniProt Accession

 NDC80_HUMAN; O14777; Q6PJX2 

Genbank Protein ID

 AF017790; BC010171 

Genbank Nucleotide ID

 AAB80726.1 

Protein Name

 Kinetochore protein NDC80 homolog 

Protein Synonyms/Alias

 Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC 

Gene Name

 NDC80 

Gene Synonyms/Alias

 HEC; HEC1; KNTC2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	LRSQDVNKQGLYTPQ	ubiquitination	[1, 2, 3]
35	GLYTPQTKEKPTFGK	ubiquitination	[3]
37	YTPQTKEKPTFGKLS	ubiquitination	[3]
47	FGKLSINKPTSERKV	ubiquitination	[3]
59	RKVSLFGKRTSGHGS	ubiquitination	[3]
89	DPRPLNDKAFIQQCI	ubiquitination	[3, 4]
123	SLQAPSVKDFLKIFT	ubiquitination	[3, 5]
127	PSVKDFLKIFTFLYG	ubiquitination	[3]
156	EEVPRIFKDLGYPFA	ubiquitination	[3]
255	AELQSKLKDLFNVDA	ubiquitination	[3]
264	LFNVDAFKLESLEAK	ubiquitination	[1, 3]
271	KLESLEAKNRALNEQ	ubiquitination	[3]
300	LESLRKLKASLQGDV	ubiquitination	[3, 4]
309	SLQGDVQKYQAYMSN	ubiquitination	[3]
327	HSAILDQKLNGLNEE	ubiquitination	[1]
346	ELECETIKQENTRLQ	ubiquitination	[3, 4]
360	QNIIDNQKYSVADIE	ubiquitination	[1, 3, 4, 6, 7]
393	DLEAEQQKLWNEELK	ubiquitination	[1, 3]
400	KLWNEELKYARGKEA	ubiquitination	[3]
405	ELKYARGKEAIETQL	ubiquitination	[1, 3]
417	TQLAEYHKLARKLKL	ubiquitination	[3]
433	PKGAENSKGYDFEIK	ubiquitination	[3]
452	AGANCLVKYRAQVYV	ubiquitination	[3]
462	AQVYVPLKELLNETE	ubiquitination	[1, 3]
504	KRSVRTLKEEVQKLD	ubiquitination	[3]
517	LDDLYQQKIKEAEEE	acetylation	[8]
527	EAEEEDEKCASELES	acetylation	[9]
606	HLEEQIAKVDREYEE	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. 

Sequence Annotation

 REGION 1 445 Interaction with the N-terminus of CDCA1.
 REGION 1 250 Nuclear localization.
 REGION 128 251 Interaction with RB1.
 REGION 251 618 Interaction with NEK2 and ZWINT.
 REGION 251 431 Interaction with SMC1A.
 REGION 361 547 Interaction with PSMC2 and SMC1A.
 REGION 446 642 Interaction with the C-terminus of CDCA1
 MOD_RES 69 69 Phosphoserine.
 MOD_RES 165 165 Phosphoserine; by NEK2.  

Keyword

 3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 642 AA 

Protein Sequence

Gene Ontology

 GO:0000942; C:condensed nuclear chromosome outer kinetochore; IDA:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031262; C:Ndc80 complex; IDA:UniProtKB.
 GO:0008608; P:attachment of spindle microtubules to kinetochore; IMP:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000132; P:establishment of mitotic spindle orientation; IMP:UniProtKB.
 GO:0000070; P:mitotic sister chromatid segregation; TAS:ProtInc.
 GO:0007052; P:mitotic spindle organization; IMP:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; NAS:UniProtKB. 

Interpro

 IPR005550; Kinetochore_Ndc80. 

Pfam

 PF03801; Ndc80_HEC 

SMART

  

PROSITE

  

PRINTS