UniProt Accession

 WDR48_HUMAN; Q8TAF3; B4DM86; B4DQI2; B4DY84; Q63HJ2; Q658Y1; Q8N3Z1; Q9NSK8; Q9P279 

Genbank Protein ID

 AF468833; AB040882; AK297349; AK298810; AK302307; AL162064; AL832926; BX649170; BC026353; BC037168 

Genbank Nucleotide ID

 AAL78650.1; BAA95973.2; BAG59798.1; BAG60944.1; BAG63646.1; CAB82402.1; CAH56300.1; CAH56182.1; AAH26353.1; AAH37168.1 

Protein Name

 WD repeat-containing protein 48 

Protein Synonyms/Alias

 USP1-associated factor 1; WD repeat endosomal protein; p80 

Gene Name

 WDR48 

Gene Synonyms/Alias

 KIAA1449; UAF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
63	IWSVNQHKQDPYIAS	ubiquitination	[1]
106	VKVWNAHKGFCMSTL	ubiquitination	[2, 3]
121	RTHKDYVKALAYAKD	acetylation	[4]
214	KGHTDNVKALLLNRD	acetylation	[4]
214	KGHTDNVKALLLNRD	ubiquitination	[5]
275	YSGGRDRKIYCTDLR	ubiquitination	[5, 6]
294	RVLICEEKAPVLKME	ubiquitination	[5, 6]
299	EEKAPVLKMELDRSA	ubiquitination	[5]
318	AIWVATTKSTVNKWT	ubiquitination	[5, 6, 7]
327	TVNKWTLKGIHNFRA	ubiquitination	[2, 3, 5, 7]
358	TQPDQVIKGGASIIQ	ubiquitination	[5, 8, 9]
372	QCHILNDKRHILTKD	ubiquitination	[5, 8, 9]
391	VAYWDVLKACKVEDL	ubiquitination	[5]
578	PHASSGAKTLKKDRL	acetylation	[4]
578	PHASSGAKTLKKDRL	ubiquitination	[2, 3, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1. 

Sequence Annotation

 REPEAT 28 67 WD 1.
 REPEAT 73 112 WD 2.
 REPEAT 115 154 WD 3.
 REPEAT 166 205 WD 4.
 REPEAT 208 247 WD 5.
 REPEAT 250 289 WD 6.
 REPEAT 292 334 WD 7.
 REPEAT 358 397 WD 8.
 MOD_RES 28 28 Phosphotyrosine (By similarity).
 MOD_RES 214 214 N6-acetyllysine.
 MOD_RES 338 338 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Host-virus interaction; Lysosome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 677 AA 

Protein Sequence

Gene Ontology

 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016579; P:protein deubiquitination; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR021772; DUF3337.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF11816; DUF3337
 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.