UniProt Accession

 VPS20_YEAST; Q04272; D6VZQ1 

Genbank Protein ID

 Z49259; BK006946 

Genbank Nucleotide ID

 CAA89223.1; DAA09975.1 

Protein Name

 Vacuolar protein sorting-associated protein 20 

Protein Synonyms/Alias

 Amino acid sensor-independent protein 10; Charged multivesicular body protein 6; ESCRT-III complex subunit VPS20; Vacuolar protein-targeting protein 20 

Gene Name

 VPS20 

Gene Synonyms/Alias

 ASI10; CHM6; VPT20; YMR077C; YM9582.02C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
200	QGEQTEQKEREEFAT	ubiquitination	[1]
214	TEERSDTKEPLALLS	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT- III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,- II and -III complex assemblies. Required for the oligomerization of SNF7 into a membrane-associated filament. The VPS20-SNF7 subcomplex is responsible for the membrane association of the ESCRT-III complex. Also required for the RIM101 repressor proteolytic activation. 

Sequence Annotation

 LIPID 2 2 N-myristoyl glycine.  

Keyword

 Coiled coil; Complete proteome; Endosome; Lipoprotein; Membrane; Myristate; Protein transport; Reference proteome; Transport; Vacuole. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0000815; C:ESCRT III complex; IDA:SGD.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0070676; P:intralumenal vesicle formation; IMP:SGD.
 GO:0045324; P:late endosome to vacuole transport; IPI:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IC:SGD. 

Interpro

 IPR005024; Snf7. 

Pfam

 PF03357; Snf7 

SMART

  

PROSITE

  

PRINTS