CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017251
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromosome alignment-maintaining phosphoprotein 1 
Protein Synonyms/Alias
 Zinc finger protein 828 
Gene Name
 Champ1 
Gene Synonyms/Alias
 D8Ertd457e; Kiaa1802; Zfp828; Znf828 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
506KVASDIWKPVLSIDAacetylation[1]
546ALFPESRKHVFLPELacetylation[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore (By similarity). 
Sequence Annotation
 ZN_FING 728 750 C2H2-type.
 REGION 261 479 Mediates interaction with MAD2L2 (By
 REGION 440 580 Mediates localization to the spindle and
 REGION 581 802 Mediates localization to the chromosome
 MOD_RES 108 108 Phosphoserine (By similarity).
 MOD_RES 204 204 Phosphoserine (By similarity).
 MOD_RES 207 207 Phosphoserine (By similarity).
 MOD_RES 237 237 Phosphoserine (By similarity).
 MOD_RES 243 243 Phosphoserine (By similarity).
 MOD_RES 265 265 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine (By similarity).
 MOD_RES 276 276 Phosphoserine (By similarity).
 MOD_RES 298 298 Phosphoserine (By similarity).
 MOD_RES 309 309 Phosphoserine.
 MOD_RES 334 334 Phosphoserine (By similarity).
 MOD_RES 345 345 Phosphoserine (By similarity).
 MOD_RES 375 375 Phosphoserine (By similarity).
 MOD_RES 394 394 Phosphoserine (By similarity).
 MOD_RES 405 405 Phosphoserine (By similarity).
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 421 421 Phosphoserine (By similarity).
 MOD_RES 425 425 Phosphoserine (By similarity).
 MOD_RES 432 432 Phosphoserine (By similarity).
 MOD_RES 434 434 Phosphoserine (By similarity).
 MOD_RES 441 441 Phosphoserine (By similarity).
 MOD_RES 448 448 Phosphoserine (By similarity).
 MOD_RES 451 451 Phosphoserine (By similarity).
 MOD_RES 479 479 N6-acetyllysine (By similarity).
 MOD_RES 497 497 Phosphoserine (By similarity).
 MOD_RES 532 532 Phosphoserine.
 MOD_RES 603 603 Phosphoserine.
 MOD_RES 617 617 Phosphoserine (By similarity).
 MOD_RES 622 622 Phosphoserine (By similarity).
 MOD_RES 641 641 Phosphoserine (By similarity).
 MOD_RES 642 642 Phosphoserine (By similarity).
 MOD_RES 643 643 Phosphoserine.
 MOD_RES 665 665 Phosphoserine (By similarity).
 MOD_RES 726 726 Phosphoserine (By similarity).  
Keyword
 Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MEVCQELRKP ALSLECGHCS FRGTDYENVQ LHMGSIHPEF CDDMDAGGLG KLIFYQKSAK 60
LFHCHKCFFT SKLYANVYYH ITARHAASDK WSEQPKEQPS KDTESGKSPS PPERQNPAFD 120
PAEARPTPAL PMEAQKTSPS LCPESQASGP PVLEPQGAGP LISPEPQAPC LPAEASKAAP 180
VPCPERVDPP CELPELEKPE RGPSPESVKS ALVSSKPPKH SSFADTGAAP SALSPESPVL 240
ATSPEPWGPS LSASPESRKP ARTASPEPRK PSPAESPELW KPFPAIASEP RRPTPAVSPG 300
SWKPGPPGSP RPWKSSPSAT SGPWKSSKPV QPMSPGPWKP IPSVSPGPWK PAPSMSTASW 360
KSSVSSGSWK TPPTSPESWK SGPPELRKTA LPLSPEHWKA VPPVSPELRR PGPPLSPEIR 420
SPAGSPELKK PSSSPDLWKV SPDQRKTSPA SLDFPEPQKS SCGSPPDLWK SSFIMESQKP 480
NVFSETRKHT ASGSSESPKV ASDIWKPVLS IDAEPRKSTL FPEPTKAVLP ASPEPRKRAL 540
FPESRKHVFL PELPKSAVFS DAQKAPELSE EIQLEAVDNA KCDSLAQEGL LATPKKLLDE 600
ALSPSSKKLK KDSQENSDAE LSSSEYIRAD LDTLDTKGQE SSSDQEQVDV ESIDFSKENK 660
MEMGSTEQAK NVLQFTEEKE AFISEEEIAK YMKRGKGKYY CKICCCRAMK KGAVLHHLVN 720
KHNVHSPYKC TICGKAFLLE SLLKNHVAAH GQSLLKCPRC NFESNFPRGF KKHLTHCQSR 780
HNEEVNKKLM EALESPLEEQ QI 802 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005819; C:spindle; ISS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0034501; P:protein localization to kinetochore; ISS:UniProtKB.
 GO:0035372; P:protein localization to microtubule; ISS:UniProtKB.
 GO:0031134; P:sister chromatid biorientation; ISS:UniProtKB. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS