CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006640
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual specificity mitogen-activated protein kinase kinase 2 
Protein Synonyms/Alias
 MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2 
Gene Name
 Map2k2 
Gene Synonyms/Alias
 Mek2; Mkk2; Prkmk2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
196QIMHRDVKPSNILVNubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). 
Sequence Annotation
 DOMAIN 72 369 Protein kinase.
 NP_BIND 78 86 ATP (By similarity).
 ACT_SITE 194 194 Proton acceptor (By similarity).
 BINDING 101 101 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 222 222 Phosphoserine; by RAF (By similarity).
 MOD_RES 226 226 Phosphoserine; by RAF (By similarity).
 MOD_RES 293 293 Phosphoserine (By similarity).
 MOD_RES 295 295 Phosphoserine (By similarity).
 MOD_RES 394 394 Phosphothreonine (By similarity).
 MOD_RES 396 396 Phosphothreonine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 400 AA 
Protein Sequence
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EAHLVDLQKK LEELDLDEQQ RKRLEAFLTQ 60
KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ 120
VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG 180
LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ 240
GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP 300
PGRPISGHGM DSRPAMAIFE LLDYIVNEPP PKLPSGVFSS DFQEFVNKCL IKNPAERADL 360
KLLTNHAFIK RSEGEDVDFA GWLCRTLRLK QPSTPTRTAV 400 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:RGD.
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0005769; C:early endosome; TAS:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0005925; C:focal adhesion; TAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
 GO:0009898; C:internal side of plasma membrane; IEA:Compara.
 GO:0005770; C:late endosome; TAS:UniProtKB.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0005739; C:mitochondrion; TAS:UniProtKB.
 GO:0005634; C:nucleus; TAS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0004708; F:MAP kinase kinase activity; IDA:RGD.
 GO:0043539; F:protein serine/threonine kinase activator activity; IEA:Compara.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0036289; P:peptidyl-serine autophosphorylation; IEA:Compara.
 GO:2000147; P:positive regulation of cell motility; IEA:Compara.
 GO:2000641; P:regulation of early endosome to late endosome transport; TAS:UniProtKB.
 GO:0090170; P:regulation of Golgi inheritance; TAS:UniProtKB.
 GO:0032872; P:regulation of stress-activated MAPK cascade; TAS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS