CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020380
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase 33 
Protein Synonyms/Alias
  
Gene Name
 STK33 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31VLCVCSSKTRVPPVLubiquitination[1]
100RGNFTEGKVPHIRIEubiquitination[1]
158KAGSSAVKLLEREVNubiquitination[1]
240DIVHRDLKLENIMVKubiquitination[1]
271TDFGLAVKKQSRSEAubiquitination[1]
373PAHRITAKELLDNQWubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines. 
Sequence Annotation
 DOMAIN 116 381 Protein kinase.
 NP_BIND 122 130 ATP (By similarity).
 ACT_SITE 238 238 Proton acceptor (By similarity).
 BINDING 145 145 ATP (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 514 AA 
Protein Sequence
MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS AESLISLERK 60
KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK VPHIRIENGA AIEEIYTFGR 120
ILGKGSFGIV IEATDKETET KWAIKKVNKE KAGSSAVKLL EREVNILKSV KHEHIIHLEQ 180
VFETPKKMYL VMELCEDGEL KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK 240
LENIMVKSSL IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 300
SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI SDCAKSVLKQ 360
LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE WKNNPESVEE NTTEEKNKPS 420
TEEKLKSYQP WGNVPDANYT SDEEEEKQST AYEKQFPATS KDNFDMCSSS FTSSKLLPAE 480
IKGEMEKTPV TPSQGTATKY PAKSGALSRT KKKL 514 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS