UniProt Accession

 CBX3_MOUSE; P23198; Q921C4 

Genbank Protein ID

 X56683; AJ278617; AJ278618; AJ278619 

Genbank Nucleotide ID

 CAA40012.1; CAC42944.1; CAC42944.1; CAC42944.1 

Protein Name

 Chromobox protein homolog 3 

Protein Synonyms/Alias

 Heterochromatin protein 1 homolog gamma; HP1 gamma; M32; Modifier 2 protein 

Gene Name

 Cbx3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
34	PEEFVVEKVLDRRVV	ubiquitination	[1]
113	KKRDAADKPRGFARG	acetylation	[2]
143	LMFLMKWKDSDEADL	acetylation	[3]
154	EADLVLAKEANMKCP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity). 

Sequence Annotation

 DOMAIN 30 88 Chromo 1.
 DOMAIN 121 179 Chromo 2; shadow subtype.
 MOD_RES 10 10 N6-acetyllysine (By similarity).
 MOD_RES 44 44 N6-acetyllysine (By similarity).
 MOD_RES 50 50 N6-acetyllysine (By similarity).
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 95 95 Phosphoserine.
 MOD_RES 97 97 Phosphoserine (By similarity).
 MOD_RES 99 99 Phosphoserine (By similarity).
 MOD_RES 176 176 Phosphoserine.  

Keyword

 Acetylation; Chromatin regulator; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 183 AA 

Protein Sequence

Gene Ontology

 GO:0000779; C:condensed chromosome, centromeric region; IDA:UniProtKB.
 GO:0031618; C:nuclear centromeric heterochromatin; IDA:UniProtKB.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR017984; Chromo_dom_subgr.
 IPR023780; Chromo_domain.
 IPR000953; Chromo_domain/shadow.
 IPR008251; Chromo_shadow_dom.
 IPR016197; Chromodomain-like.
 IPR023779; Chromodomain_CS. 

Pfam

 PF00385; Chromo
 PF01393; Chromo_shadow 

SMART

 SM00298; CHROMO
 SM00300; ChSh 

PROSITE

 PS00598; CHROMO_1
 PS50013; CHROMO_2 

PRINTS

 PR00504; CHROMODOMAIN.