CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012905
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ferredoxin--nitrite reductase, chloroplastic 
Protein Synonyms/Alias
 NiR 
Gene Name
 NIR1 
Gene Synonyms/Alias
 NIR; At2g15620; F9O13.17 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
103MEELDSEKSSKDDIDubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265
Functional Description
 Catalyzes the six-electron reduction of nitrite to ammonium. 
Sequence Annotation
 METAL 464 464 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 470 470 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 505 505 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 509 509 Iron (siroheme axial ligand) (By
 METAL 509 509 Iron-sulfur (4Fe-4S) (By similarity).
 CROSSLNK 103 103 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 4Fe-4S; Chloroplast; Complete proteome; Electron transport; Heme; Iron; Iron-sulfur; Isopeptide bond; Metal-binding; Nitrate assimilation; Oxidoreductase; Plastid; Reference proteome; Repeat; Transit peptide; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 586 AA 
Protein Sequence
MTSFSLTFTS PLLPSSSTKP KRSVLVAAAQ TTAPAESTAS VDADRLEPRV ELKDGFFILK 60
EKFRKGINPQ EKVKIEREPM KLFMENGIEE LAKKSMEELD SEKSSKDDID VRLKWLGLFH 120
RRKHQYGKFM MRLKLPNGVT TSAQTRYLAS VIRKYGEDGC ADVTTRQNWQ IRGVVLPDVP 180
EILKGLASVG LTSLQSGMDN VRNPVGNPIA GIDPEEIVDT RPYTNLLSQF ITANSQGNPD 240
FTNLPRKWNV CVVGTHDLYE HPHINDLAYM PANKDGRFGF NLLVGGFFSP KRCEEAIPLD 300
AWVPADDVLP LCKAVLEAYR DLGTRGNRQK TRMMWLIDEL GVEGFRTEVE KRMPNGKLER 360
GSSEDLVNKQ WERRDYFGVN PQKQEGLSFV GLHVPVGRLQ ADDMDELARL ADTYGSGELR 420
LTVEQNIIIP NVETSKTEAL LQEPFLKNRF SPEPSILMKG LVACTGSQFC GQAIIETKLR 480
ALKVTEEVER LVSVPRPIRM HWTGCPNTCG QVQVADIGFM GCLTRGEEGK PVEGADVYVG 540
GRIGSDSHIG EIYKKGVRVT ELVPLVAEIL IKEFGAVPRE REENED 586 
Gene Ontology
 GO:0048046; C:apoplast; IDA:TAIR.
 GO:0009570; C:chloroplast stroma; IDA:TAIR.
 GO:0016020; C:membrane; IDA:TAIR.
 GO:0005739; C:mitochondrion; IDA:TAIR.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0048307; F:ferredoxin-nitrite reductase activity; IEA:EC.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050421; F:nitrite reductase (NO-forming) activity; ISS:TAIR.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniPathway.
 GO:0010167; P:response to nitrate; IEP:TAIR. 
Interpro
 IPR005117; NiRdtase/SiRdtase_haem-b_fer.
 IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
 IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS. 
Pfam
 PF01077; NIR_SIR
 PF03460; NIR_SIR_ferr 
SMART
  
PROSITE
 PS00365; NIR_SIR 
PRINTS
 PR00397; SIROHAEM.