| Tag | Content |
|---|
| CPLM ID | CPLM-023719 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ARF GTPase-activating protein GIT1 |
Protein Synonyms/Alias | ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 |
Gene Name | GIT1 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 49 | GRHISIVKHLRHSAW | ubiquitination | [1] | | 135 | VTAKDLSKQLHSSVR | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. |
Sequence Annotation | DOMAIN 1 124 Arf-GAP.
REPEAT 132 161 ANK 1.
REPEAT 166 195 ANK 2.
REPEAT 199 228 ANK 3.
ZN_FING 11 34 C4-type.
REGION 251 415 PTK2/FAK1-binding (By similarity).
REGION 254 367 ARHGEF6-binding (By similarity).
REGION 637 761 Interaction with PXN and TGFB1I1 (By
MOD_RES 224 224 Phosphotyrosine (By similarity).
MOD_RES 361 361 Phosphoserine (By similarity).
MOD_RES 362 362 Phosphoserine.
MOD_RES 364 364 Phosphothreonine.
MOD_RES 375 375 Phosphoserine.
MOD_RES 383 383 Phosphotyrosine.
MOD_RES 385 385 Phosphoserine.
MOD_RES 388 388 Phosphoserine.
MOD_RES 392 392 Phosphothreonine.
MOD_RES 410 410 Phosphoserine.
MOD_RES 508 508 Phosphoserine (By similarity).
MOD_RES 545 545 Phosphotyrosine.
MOD_RES 554 554 Phosphotyrosine.
MOD_RES 592 592 Phosphoserine.
MOD_RES 596 596 Phosphoserine.
MOD_RES 601 601 Phosphothreonine. |
Keyword | Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; GTPase activation; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 761 AA |
Protein Sequence | MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH LRHSAWPPTL 60
LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH PIKSEFIRAK YQMLAFVHKL 120
PCRDDDGVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT 180
LQAELLVVYG ADPGSPDVNG RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD 240
HKNGHYIIPQ MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE 300
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA TLIIDILSEA 360
KRRQQGKSLS SPTDNLELSL RSQSDLDDQH DYDSVASDED TDQEPLRSTG ATRSNRARSM 420
DSSDLSDGAV TLQEYLELKK ALATSEAKVQ QLMKVNSSLS DELRRLQREI HKLQAENLQL 480
RQPPGPVPTP PLPSERAEHT PMAPGGSTHR RDRQAFSMYE PGSALKPFGG PPGDELTTRL 540
QPFHSTELED DAIYSVHVPA GLYRIRKGVS ASAVPFTPSS PLLSCSQEGS RHTSKLSRHG 600
SGADSDYENT QSGDPLLGLE GKRFLELGKE EDFHPELESL DGDLDPGLPS TEDVILKTEQ 660
VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK RPALEPVRSS LRLLNASAYR 720
LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA YDIAKAAKQL VTITTREKKQ 770 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:HPA. GO:0005925; C:focal adhesion; IDA:HPA. GO:0008060; F:ARF GTPase activator activity; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0043547; P:positive regulation of GTPase activity; IEA:GOC. GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro. GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; TAS:ProtInc. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |