CPLM-017351

Genbank Nucleotide ID

Mitochondrial cardiolipin hydrolase

Protein Synonyms/Alias

Choline phosphatase 6; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D6; PLD 6; Protein zucchini homolog

Homo sapiens (Human)

Position	Peptide	Type	References
158	DPGYMHHKFAIVDKR	ubiquitination	[1, 2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.

DOMAIN 151 178 PLD phosphodiesterase.
ZN_FING 45 78 C3H1-type; atypical.
REGION 1 39 Required for mitochondrial localization.
ACT_SITE 156 156 Probable.
ACT_SITE 158 158 Potential.
ACT_SITE 163 163 Potential.

Complete proteome; Differentiation; Endonuclease; Hydrolase; Lipid degradation; Lipid metabolism; Meiosis; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nuclease; Polymorphism; Reference proteome; Spermatogenesis; Transmembrane; Transmembrane helix; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
GO:0035755; F:cardiolipin hydrolase activity; IDA:UniProtKB.
GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
GO:0043046; P:DNA methylation involved in gamete generation; ISS:UniProtKB.
GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
GO:0007126; P:meiosis; ISS:UniProtKB.
GO:0008053; P:mitochondrial fusion; IMP:UniProtKB.
GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
GO:0030719; P:P granule organization; ISS:UniProtKB.
GO:0006655; P:phosphatidylglycerol biosynthetic process; TAS:Reactome.
GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.
GO:0007286; P:spermatid development; ISS:UniProtKB.

IPR025202; PLD-like_dom.
IPR001736; PLipase_D/transphosphatidylase.

PS50035; PLD
PS50103; ZF_C3H1