| Tag | Content |
|---|
| CPLM ID | CPLM-004317 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphoribosylformylglycinamidine synthase |
Protein Synonyms/Alias | FGAM synthase; FGAMS; Formylglycinamide ribotide amidotransferase; FGARAT; Formylglycinamide ribotide synthetase |
Gene Name | purL |
Gene Synonyms/Alias | purI; b2557; JW2541 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 60 | AQLERLLKYGPALAS | acetylation | [1] | | 73 | ASHAPQGKLLLVTPR | acetylation | [1] | | 220 | NSEHCRHKIFNADWV | acetylation | [1] | | 235 | IDGEQQPKSLFKMIK | acetylation | [1] | | 239 | QQPKSLFKMIKNTFE | acetylation | [1] | | 242 | KSLFKMIKNTFETTP | acetylation | [1] | | 257 | DHVLSAYKDNAAVME | acetylation | [1] | | 292 | EPAHILMKVETHNHP | acetylation | [1] | | 355 | PWEEDFGKPERIVTA | acetylation | [1] | | 395 | YFRTYEEKVNSHNGE | acetylation | [1] | | 409 | EELRGYHKPIMLAGG | acetylation | [1] | | 524 | SDGGRGGKFELREIL | acetylation | [1] | | 568 | PLFDELCKRERAPYA | acetylation | [1] | | 608 | PLDVLLGKTPKMTRD | acetylation | [1] | | 638 | ITIADAVKRVLHLPT | acetylation | [1] | | 732 | ATQIGDIKRIKLSAN | acetylation | [1] | | 776 | GLTIPVGKDSMSMKT | acetylation | [1] | | 853 | VYRQLGDKPADVRDV | acetylation | [1] | | 1215 | HLAALESKGLVALRY | acetylation | [1] | | 1228 | RYVDNFGKVTETYPA | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | |
Sequence Annotation | DOMAIN 1042 1295 Glutamine amidotransferase type-1.
NP_BIND 307 318 ATP (Potential).
ACT_SITE 1135 1135 Nucleophile (By similarity).
ACT_SITE 1260 1260 By similarity.
ACT_SITE 1262 1262 By similarity. |
Keyword | ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glutamine amidotransferase; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1295 AA |
Protein Sequence | MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK 60
YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT 120
NEQWQQVTAE LHDRMMETVF FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL 180
ALAEDEIDYL QDAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWVIDG EQQPKSLFKM 240
IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT 300
AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 360
TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI 420
RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR 480
CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELREI LSDEPGMSPL 540
EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL 600
PLDVLLGKTP KMTRDVQTLK AKGDALAREG ITIADAVKRV LHLPTVAEKT FLVTIGDRSV 660
TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER APVALLDFAA SARLAVGEAL 720
TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 780
MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA 840
TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA 900
GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS 960
GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK 1020
LSFDINEDVA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLTGRT 1080
GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM 1140
MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 1200
VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH 1260
PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG 1295 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:EcoliWiki. GO:0005524; F:ATP binding; IDA:EcoliWiki. GO:0042623; F:ATPase activity, coupled; IDA:EcoliWiki. GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IDA:EcoCyc. GO:0006189; P:'de novo' IMP biosynthetic process; IC:EcoliWiki. GO:0006541; P:glutamine metabolic process; IDA:EcoliWiki. |
Interpro | |
Pfam | |
SMART | |
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PRINTS | |